Browsing División de Biología Molecular by Author "Balderas Hernández, Víctor Emmanuel"
Now showing items 1-18 of 18
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Agave salmiana syrup improves the production of recombinant human interleukin-2 in Escherichia coli
Balderas Hernández, Víctor Emmanuel; Medina Rivero, Emilio; Barba de la Rosa, Ana Paulina; De León Rodríguez, Antonio (Universidad Autónoma Metropolitana, 2021)"The expression of heterologous proteins in Escherichia coli is strongly affected by the type of carbon source used. In this work, the expression of a synthetic codon-optimized gene of human interleukin-2 in E. coli BL21-SI, ... -
Autodisplay de una xilanasa en células completas para la degradación de hemicelulosa y obtención de biocombustibles
Salas Montantes, Carlos Joel (2017-12)"Para mitigar los efectos ambientales causados por el uso de combustibles fósiles, se planea impulsar el uso de biocombustibles. Actualmente su producción se basa en la fermentación de carbohidratos obtenidos de cultivos ... -
Autodisplay of an endo-1,4-?-xylanase from Clostridium cellulovorans in Escherichia coli for xylans degradation
Balderas Hernández, Víctor Emmanuel; Salas Montantes, Carlos Joel; Barba de la Rosa, Ana Paulina; De León Rodríguez, Antonio (Elsevier, 2021)"In this work, the scale-up from 0.01 to 10 L process for the co-production of hydrogen (H2) and ethanol (EtOH) by a genetically engineered Escherichia coli that utilizes hemicellulosic hydrolysates from wheat straw ... -
Biodegradation of diisononyl phthalate by a consortium of saline soil bacteria: optimisation and kinetic characterisation
Pereyra Camacho, Marco Antonio; Balderas Hernández, Víctor Emmanuel; De León Rodríguez, Antonio (Springer, 2021)"Diisononyl phthalate (DINP) is one of plasticisers most employed in the production of plastic materials and belongs to the most important environmental contaminants. In this work, a consortium of saline soil bacterial ... -
Biohydrogen production by the psychrophilic G088 strain using single carbohydrates as substrate
Alvarez Guzmán, Cecilia Lizeth; Oceguera Contreras, Edén; Ornelas Salas, José Tomás; Balderas Hernández, Víctor Emmanuel; De León Rodríguez, Antonio (Elsevier, 2016-05)"The production of biohydrogen by psychrophilic G088 strain ([EU636029]), closely related to Polaromonas rhizosphaerae ([EF127651]) was evaluated using xylose, glucose, fructose, galactose, lactose or sucrose as a carbon ... -
Biohydrogen production from cheese whey powder by Enterobacter asburiae: Effect of operating conditions on hydrogen yield and chemometric study of the fermentative metabolites
Alvarez Guzmán, Cecilia Lizeth; Cisneros de la Cueva, Sergio; Balderas Hernández, Víctor Emmanuel; Smoliski, Adam; De León Rodríguez, Antonio (Elsevier, 2020)"In this study, the response surface methodology (RSM) with a central composite design (CCD) was applied to evaluate the effect of temperature, initial pH and cheese whey powder concentration (CWP) on the hydrogen yield ... -
Constitutive expression of the active fragment of human vasostatin Vs30 in Pichia pastoris SMD1168H
Calderón Salais, Sergio; Velázquez Bernardino, Prisiliana; Balderas Hernández, Víctor Emmanuel; Barba de la Rosa, Ana Paulina; De León Rodríguez, Antonio (Elsevier, 2018)"Vasostatin 30 (Vs30) is an active fragment derived from the N-terminal region (135–164 aa) of human calreticulin and has the ability to inhibit angiogenesis. In this work, the expression of Vs30 was performed using a ... -
Coproduction of hydrogen, ethanol and 2,3-butanediol from agro-industrial residues by the Antarctic psychrophilic GA0F bacterium
Alvarez Guzmán, Cecilia Lizeth; Balderas Hernández, Víctor Emmanuel; De León Rodríguez, Antonio (Elsevier, 2020)"In this study, the simultaneous production of hydrogen, ethanol, and 2,3-butanediol was assessed using three agro-industrial residues: cheese whey powder (CWP), wheat straw hydrolysate (WSH) and sugarcane molasses (SCM), ... -
Diminution of migration of phthalic acid esters in tequila beverage by the year of production
Balderas Hernández, Víctor Emmanuel; Ornelas Salas, José Tomás; Barba de la Rosa, Ana Paulina; De León Rodríguez, Antonio (Taylor & Francis, 2020)"The presence of diethyl-phthalate (DEP), dibutyl-phthalate (DBP), butylbenzyl-phthalate (BBP), diethylhexyl-phthalate (DEHP) and diisononyl-phthalate (DINP) was determined in 295 tequila samples. They were grouped by age ... -
Diseño de un sistema de expresión de proteínas recombinantes del virus de influenza A (H7N9) en Bacillus subtilis
Pereyra Camacho, Marco Antonio (2016)"La influenza es una enfermedad infecciosa provocada por el virus de la influenza, sus principales hospederos son las aves silvestres, sin embargo, puede infectar personas, aves de corral, cerdos y otros animales. Actualmente ... -
Inactivation of the transcription factor mig1 (YGL035C) in Saccharomyces cerevisiae improves tolerance towards monocarboxylic weak acids: acetic, formic and levulinic acid
Balderas Hernández, Víctor Emmanuel; Correia, Kevin; Mahadevan, Radhakrishnan (Springer, 2018)"Toxic concentrations of monocarboxylic weak acids present in lignocellulosic hydrolyzates affect cell integrity and fermentative performance of Saccharomyces cerevisiae. In this work, we report the deletion of the general ... -
Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-γ but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli
Medina Rivero, Emilio; Balderas Hernández, Víctor Emmanuel; Ordoñez Acevedo, Leandro Gabriel; Paz Maldonado, Luz María Teresita; Barba de la Rosa, Ana Paulina; De León Rodríguez, Antonio (2007-09)"Production of periplasmic human interferon-γ (hINF-γ) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which ... -
Optimization of biohydrogen production by the novel psychrophilic strain N92 collected from the Antarctica
Cisneros de la Cueva, Sergio; Alvarez Guzmán, Cecilia Lizeth; Balderas Hernández, Víctor Emmanuel; De León Rodríguez, Antonio (Elsevier, 2018)"In this study, the response surface methodology (RSM) with central composite design (CCD) was employed to improve the hydrogen production by the psychrophilic N92 strain (EU636058) isolated from Antarctica, which is closely ... -
Optimization of culture conditions for a synthetic gene expression in Escherichia coli using response surface methodology: the case of human interferon beta
Paz Maldonado, Luz María Teresita; Balderas Hernández, Víctor Emmanuel; Medina Rivero, Emilio; Barba de la Rosa, Ana Paulina; Flores Flores, José Luis; Ordoñez Acevedo, Leandro Gabriel; De León Rodríguez, Antonio (Elsevier, 2007-06)"A human interferon beta (hINF-β) synthetic gene was optimized and expressed in Escherichia coli BL21-SI using a vector with the T7 promoter. To determine the best culture conditions such as culture medium, temperature, ... -
Optimization of human interferon gamma production in Escherichia coli by response surface methodology
Balderas Hernández, Víctor Emmanuel; Paz Maldonado, Luz María Teresita; Medina Rivero, Emilio; Barba de la Rosa, Ana Paulina; Ordoñez Acevedo, Leandro Gabriel; De León Rodríguez, Antonio (2008-02)"The production of human interferon gamma (hIFN-γ) using a synthetic gene in Escherichia coli BL21-SI was optimized by response surface methodology (RSM) and a Box-Behnken design. The process variables studied were ... -
Optimization of hydrogen production by the psychrophilic strain G088
Alvarez Guzmán, Cecilia Lizeth (2016-07)"Hydrogen (H2) is considered as one of the most promising energy carriers for the future, it has several unique characteristics, such as high energy yield, it is renewable, clean and its combustion is sustainable and ... -
Scale-up of hydrogen and ethanol co-production by an engineered Escherichia coli
López Hidalgo, Angel Mario; Balderas Hernández, Víctor Emmanuel; De León Rodríguez, Antonio (Elsevier, 2021)"In this work, the scale-up from 0.01 to 10 L process for the co-production of hydrogen (H2) and ethanol (EtOH) by a genetically engineered Escherichia coli that utilizes hemicellulosic hydrolysates from wheat straw ... -
Sistema para expresar y transportar proteinas recombinantes al periplasma de escherichid coli por la vía de secreción Tat
Barba de la Rosa, Ana Paulina; Medina Rivero, Emilio; Ordoñez Acevedo, Leandro Gabriel; Paz Maldonado, Luz María Teresita; Balderas Hernández, Víctor Emmanuel; De León Rodríguez, Antonio (2015-07)"La presente invención describe un vector de expresión que contiene un péptido señal que utiliza la vía de secreción Tat como alternativa al sistema Sec de secreción para el transporte de citocininas y otras proteínas de ...