Listar División de Biología Molecular por autor "Ordoñez Acevedo, Leandro Gabriel"
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Analysis of phthalic acid, bisphenol A and bisphenol A dimethacrylate in Mexican food cans by HPLC with evaporative light scattering detector
González Castro, Martha Irene; Ordoñez Acevedo, Leandro Gabriel; Herrera Vázquez, Yanoula; Ramírez Telles, Jorge Alberto; De León Rodríguez, Antonio (2013-06)"Bisphenol A (BPA), bisphenol A dimethacrylate (BisDMA) and phthalic acid (PA) endocrine disruptors can migrate from the plastic lining of cans to foods producing serious health problems when they exceed allowable concentration ... -
Characterization of volatile compounds from ethnic Agave alcoholic beverages by gas chromatography-mass spectrometry
De León Rodríguez, Antonio; Escalante Minakata, María del Pilar; Jiménez García, María Isabel; Ordoñez Acevedo, Leandro Gabriel; Flores Flores, José Luis; Barba de la Rosa, Ana Paulina (Faculty of Food Technology and Biotechnology, University of Zagreb, 2008)"Ethnic Agave alcoholic beverages such as raicilla, sisal, tequila, mezcal, bacanora, sotol and pulque have been analyzed by gas chromatography and headspace solid-phase microextraction- gas chromatography-mass spectrometry ... -
Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica
García Echauri, Sergio Ángel; Ordoñez Acevedo, Leandro Gabriel; Rojas Contreras, Juan Antonio; Barba de la Rosa, Ana Paulina; De León Rodríguez, Antonio (Elsevier, 2009-09)"Superoxide dismutase (SOD) catalyzes the conversion of the superoxide radical (O2−) into oxygen and hydrogen peroxide. Deschampsia antarctica is a plant that grows in Antarctica and survives to extreme low temperature and ... -
Hydrogen production by Escherichia coli ΔhycA ΔlacI using cheese whey as substrate
Rosales Colunga, Luis Manuel; Razo Flores, Elías; Ordoñez Acevedo, Leandro Gabriel; Alatriste Mondragón, Felipe; De León Rodríguez, Antonio (Elsevier, 2010-01)"This study reports a fermentative hydrogen production by Escherichia coli using cheese whey as substrate. To improve the biohydrogen production, an E. coli ΔhycA ΔlacI strain (WDHL) was constructed. The absence of hycA ... -
Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-γ but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli
Medina Rivero, Emilio; Balderas Hernández, Víctor Emmanuel; Ordoñez Acevedo, Leandro Gabriel; Paz Maldonado, Luz María Teresita; Barba de la Rosa, Ana Paulina; De León Rodríguez, Antonio (2007-09)"Production of periplasmic human interferon-γ (hINF-γ) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which ... -
Nitrogen sources impact hydrogen production by Escherichia coli using cheese whey as substrate
Alvarado Cuevas, Zazil Donaxí; Ordoñez Acevedo, Leandro Gabriel; Ornelas Salas, José Tomás; De León Rodríguez, Antonio (Elsevier, 2013-09)"The impact of nitrogen source on hydrogen production by Escherichia coli WDHL (Delta hycA Delta lacI) strain using cheese whey as a substrate was evaluated. To improve the assimilation of complex proteins such as lactalbumin, ... -
Optimization of culture conditions for a synthetic gene expression in Escherichia coli using response surface methodology: the case of human interferon beta
Paz Maldonado, Luz María Teresita; Balderas Hernández, Víctor Emmanuel; Medina Rivero, Emilio; Barba de la Rosa, Ana Paulina; Flores Flores, José Luis; Ordoñez Acevedo, Leandro Gabriel; De León Rodríguez, Antonio (Elsevier, 2007-06)"A human interferon beta (hINF-β) synthetic gene was optimized and expressed in Escherichia coli BL21-SI using a vector with the T7 promoter. To determine the best culture conditions such as culture medium, temperature, ... -
Optimization of human interferon gamma production in Escherichia coli by response surface methodology
Balderas Hernández, Víctor Emmanuel; Paz Maldonado, Luz María Teresita; Medina Rivero, Emilio; Barba de la Rosa, Ana Paulina; Ordoñez Acevedo, Leandro Gabriel; De León Rodríguez, Antonio (2008-02)"The production of human interferon gamma (hIFN-γ) using a synthetic gene in Escherichia coli BL21-SI was optimized by response surface methodology (RSM) and a Box-Behnken design. The process variables studied were ... -
Phthalates and bisphenols migration in Mexican food cans and plastic food containers
González Castro, Martha Irene; Olea Serrano, Fatima; Rivas Velasco, Ana; Medina Rivero, Emilio; Ordoñez Acevedo, Leandro Gabriel; De León Rodríguez, Antonio (2011-06)"The presence of endocrine disruptors bisphenol-A, bisphenol-A-dimethacrylate, bisphenol-A-diglycidyl-ether, phthalic-acid, dibutyl-phthalate, diethyl-phthalate and dioctyl-phthalate was determined in vegetable cans, baby ... -
Proceso para producción simultanea de biohidrógeno y bioetanol utilizando hidrolizados lignocelulosicos como sustrato
De León Rodríguez, Antonio; Alvarado Cuevas, Zazil Donaxí; Ornelas Salas, José Tomás; López Hidalgo, Angel Mario; Ordoñez Acevedo, Leandro Gabriel (2010-08)"La presente invención describe un proceso de producción simultánea de biohidrógeno y bioetanol por una cepa de Escherichia coli que comprende al menos una deleción completa del gen hycA utilizando un sustrato que comprende ... -
Replicative and integrative plasmids for production of human interferon gamma in Bacillus subtilis
Rojas Contreras, Juan Antonio; Pedraza Reyes, Mario; Ordoñez Acevedo, Leandro Gabriel; Urtiz Estrada, Norma; Barba de la Rosa, Ana Paulina; De León Rodríguez, Antonio (2010-11)"Integrative and replicative plasmids for the expression driven by the P43 promoter and secretion of recombinant proteins in Bacillus subtilis were constructed. The plasmids named pInt and pRep respectively were tested for ... -
Sistema para expresar y transportar proteinas recombinantes al periplasma de escherichid coli por la vía de secreción Tat
Barba de la Rosa, Ana Paulina; Medina Rivero, Emilio; Ordoñez Acevedo, Leandro Gabriel; Paz Maldonado, Luz María Teresita; Balderas Hernández, Víctor Emmanuel; De León Rodríguez, Antonio (2015-07)"La presente invención describe un vector de expresión que contiene un péptido señal que utiliza la vía de secreción Tat como alternativa al sistema Sec de secreción para el transporte de citocininas y otras proteínas de ...