División de Biología Molecular: Envíos recientes
Mostrando ítems 141-160 de 602
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Genotyping based on thermal denaturation of amplification products identifies species of the Mycobacterium tuberculosis complex
(Microbiology Society, 2018)"Purpose. To develop a fast and inexpensive genotyping assay to identify the Mycobacterium tuberculosis complex (MTC) species most prevalent in human tuberculosis (TB), based on the thermal denaturation profiles of PCR ... -
Generation of Sporothrix schenckii mutants expressing the green fluorescent protein suitable for the study of host-fungus interactions
(Elsevier, 2018)"Sporotrichosis is an infection caused by members of the Sporothrix genus, and among them, Sporothrix schenckii is one of the etiological agents. Both, the disease and the causative agent have gained interest in the recent ... -
Down-regulation of arginine decarboxylase gene-expression results in reactive oxygen species accumulation in Arabidopsis
(Elsevier, 2018)"Arabidopsis amiR:ADC-L2 is a non-lethal line with several developmental defects, it is characterized by a drastic reduction in free polyamine content. Herein, we found that catalase application had growth-promoting effects ... -
Phenolic compounds accumulation in wild and domesticated cladodes from opuntia spp. and its benefits in cardiovascular diseases
(American Chemical Society, 2018)"Opuntia spp. are plants native from Mexico where the largest varieties of wild and semi-domesticated species are found. Nowadays the most domesticated species, O. ficus-indica is widely distributed worldwide. Since ... -
Anthelmintic effect of Psidium guajava and Tagetes erecta on wild-type and Levamisole-resistant Caenorhabditis elegans strains
(Elsevier, 2017)"Ethnopharmacological relevance Psidium guajava and Tagetes erecta have been used traditionally to treat gastrointestinal parasites, but their active metabolites and mechanisms of action remain largely unknown. Aim of ... -
Optimization of biohydrogen production by the novel psychrophilic strain N92 collected from the Antarctica
(Elsevier, 2018)"In this study, the response surface methodology (RSM) with central composite design (CCD) was employed to improve the hydrogen production by the psychrophilic N92 strain (EU636058) isolated from Antarctica, which is closely ... -
Chromatin loop formation induced by a subtelomeric protosilencer represses EPA genes in Candida glabrata
(Genetics Society of America, 2018)"Adherence, an important virulence factor, is mediated by the EPA (Epithelial Adhesin) genes in the opportunistic pathogen Candida glabrata. Expression of adhesin-encoding genes requires tight regulation to respond to harsh ... -
Diversification of transcriptional regulation determines subfunctionalization of paralogous branched chain aminotransferases in the yeast Saccharomyces cerevisiae
(Genetics Society of America, 2017)"Saccharomyces cerevisiae harbors BAT1 and BAT2 paralogous genes that encode branched chain aminotransferases and have opposed expression profiles and physiological roles . Accordingly, in primary nitrogen sources such as ... -
Candida glabrata encodes a longer variant of the mating type (MAT) alpha2 gene in the mating type-like MTL3 locus, which can form homodimers
(Oxford University Press, 2016)"The fungal pathogen Candida glabrata is a haploid asexual yeast. Candida glabrata contains orthologs of the genes that control mating and cell-type identity in other fungi, which encode putative transcription factors ... -
Bacterial wilt and canker of tomato: fundamentals of a complex biological system
(Springer, 2018)"Tomato (Solanum lycopersicum) is well-known as a model for study of plant–pathogen interactions, since it is a crop of global relevance and susceptible to multiple bacterial, fungal, viral and nematode pathogens. Among ... -
Physiological concentrations of zinc have dual effects on P2X myenteric receptors of guinea pig
(Springer, 2018)"We, hereby, characterize the pharmacological effects of physiological concentrations of Zinc on native myenteric P2X receptors from guinea-pig small intestine and on P2X2 isoforms present in most myenteric neurons. This ... -
Biohydrogen production from mixtures of agro-industrial wastes: Chemometric analysis, optimization and scaling up
(Elsevier, 2018)"Cheese whey (CW) and wheat straw hydrolysate (WSH) were used to produce biohydrogen by anaerobic co-digestion of multiple substrates. In this work, the influence of pH, temperature, substrates concentrations on the ... -
Constitutive expression of the active fragment of human vasostatin Vs30 in Pichia pastoris SMD1168H
(Elsevier, 2018)"Vasostatin 30 (Vs30) is an active fragment derived from the N-terminal region (135–164 aa) of human calreticulin and has the ability to inhibit angiogenesis. In this work, the expression of Vs30 was performed using a ... -
Recreating intestinal peristalsis in the petri dish
(Humana Press Inc, 2017)"Here we describe a culture technique of cells dissociated from the external muscularis of the guinea pig small intestine, which allows us to maintain all the elements involved in the intestinal peristaltic reflex. After ... -
A pilot study applying the plant Anchored Hybrid Enrichment method to New World sages (Salvia subgenus Calosphace; Lamiaceae)
(Elsevier, 2017)"We conducted a pilot study using Anchored Hybrid Enrichment to resolve relationships among a mostly Neotropical sage lineage that may have undergone a recent evolutionary radiation. Conventional markers (ITS, trnL-trnF ... -
Multilocus coalescent species delimitation to evaluate traditionally defined morphotypes in Hydrangea sect. Asperae (Hydrangeaceae)
(Elsevier, 2017)"The number of species recognized in section Asperae of the flowering plant genus Hydrangea differs widely between subsequent revisions. This variation is largely centered around the H. aspera species complex, with numbers ... -
Inactivation of the transcription factor mig1 (YGL035C) in Saccharomyces cerevisiae improves tolerance towards monocarboxylic weak acids: acetic, formic and levulinic acid
(Springer, 2018)"Toxic concentrations of monocarboxylic weak acids present in lignocellulosic hydrolyzates affect cell integrity and fermentative performance of Saccharomyces cerevisiae. In this work, we report the deletion of the general ... -
A competent catalytic active site is necessary for substrate induced dimer assembly in triosephosphate isomerase
(Elsevier, 2017)"The protozoan parasite Trichomonas vaginalis contains two nearly identical triosephosphate isomerases (TvTIMs) that dissociate into stable monomers and dimerize upon substrate binding. Herein, we compare the role of the ... -
Farmacogenética de las reacciones cutáneas adversas a lamotrigina
(2019-08)"Contexto. Las reacciones cutáneas adversas (RCA) a fármacos antiepilépticos (FAE) incluyen el exantema maculopapular, el síndrome de Stevens-Johnson y la necrólisis epidérmica tóxica y son un problema de salud pública y ... -
Expresión de una proteína de fusión vasostatina 30 y vasoinhibina 14.1 (Osiris) en Pichia pastoris y evaluación de la actividad biológica de la Vasostatina 30 en pez cebra.
(2019-08-10)"El proceso angiogénico consiste en la formación de nuevos vasos sanguíneos a partir de los preexistentes y son clave en el crecimiento y metástasis del cáncer; estos proveen oxígeno y nutrientes que permiten el crecimiento ...