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Phylogenetic diversity of methyl-coenzyme M reductase (mcrA) gene and methanogenesis from trimethylamine in hypersaline environments

dc.contributor.authorGarcía Maldonado, José Quinatzín
dc.contributor.authorCelis García, María de Lourdes Berenice
dc.contributor.authorLópez Cortés, Alejandro
dc.date.accessioned2018-03-22T23:17:35Z
dc.date.available2018-03-22T23:17:35Z
dc.date.issued2012
dc.identifier.citationGarcía-Maldonado JQ, Bebout BM, Celis LB & López-Cortés A.. (2012). Phylogenetic diversity of methyl-coenzyme M reductase (mcrA) gene and methanogenesis from trimethylamine in hypersaline environments.. International Microbiology, 15, 33-41 https://doi.org/10.2436/20.1501.01.155
dc.identifier.urihttp://hdl.handle.net/11627/3567
dc.description.abstract"Methanogens have been reported in complex microbial communities from hypersaline environments, but little is known about their phylogenetic diversity. In this work, methane concentrations in environmental gas samples were determined while methane production rates were measured in microcosm experiments with competitive and non-competitive substrates. In addition, the phylogenetic diversity of methanogens in microbial mats from two geographical locations was analyzed: the well studied Guerrero Negro hypersaline ecosystem, and a site not previously investigated, namely Laguna San Ignacio, Baja California Sur, Mexico. Methanogenesis in these microbial mats was suspected based on the detection of methane (in the range of 0.00086 to 3.204 %) in environmental gas samples. Microcosm experiments confirmed methane production by the mats and demonstrated that it was promoted only by non-competitive substrates (trimethylamine and methanol), suggesting that methylotrophy is the main characteristic process by which these hypersaline microbial mats produce methane. Phylogenetic analysis of amino acid sequences of the methyl coenzyme-M reductase (mcrA) gene from natural and manipulated samples revealed various methylotrophic methanogens belonging exclusively to the family Methanosarcinaceae. Moderately halophilic microorganisms of the genus Methanohalophilus were predominant (>60 % of mcrA sequences retrieved). Slightly halophilic and marine microorganisms of the genera Methanococcoides and Methanolobus, respectively, were also identified, but in lower abundances."
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectMethanosarcinaceae
dc.subjectHypersaline environments
dc.subjectMicrobial mats
dc.subjectTrimethylamine
dc.subjectGene mcrA
dc.subject.classificationCIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA
dc.titlePhylogenetic diversity of methyl-coenzyme M reductase (mcrA) gene and methanogenesis from trimethylamine in hypersaline environments
dc.typearticle
dc.identifier.doihttps://doi.org/10.2436/20.1501.01.155
dc.rights.accessAcceso Abierto


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional