Show simple item record


yKu70/yKu80 and Rif1 regulate silencing differentially at telomeres in Candida glabrata

dc.contributor.authorRosas Hernández, Lluvia Leticia
dc.contributor.authorJuárez Reyes, Alejandro
dc.contributor.authorArroyo Helguera, Omar Elind
dc.contributor.authorDe Las Peñas Nava, Alejandro
dc.contributor.authorCastaño Navarro, Irene Beatriz
dc.contributor.editorAmerican Society for Microbiology
dc.identifier.citationRosas-Hernández LL, Juárez-Reyes A, Arroyo-Helguera OE, De Las Peñas A, Pan SJ, Cormack BP, & Castaño I.. (2008). yKu70/yKu80 and Rif1 Regulate Silencing Differentially at Telomeres in Candida glabrata. Eukaryotic Cell, 7, 2168-2178.
dc.description.abstract"Candida glabrata, a common opportunistic fungal pathogen, adheres efficiently to mammalian epithelial cells in culture. This interaction in vitro depends mainly on the adhesin Epa1, one of a large family of cell wall proteins. Most of the EPA genes are located in subtelomeric regions, where they are transcriptionally repressed by silencing. In order to better characterize the transcriptional regulation of the EPA family, we have assessed the importance of C. glabrata orthologues of known regulators of subtelomeric silencing in Saccharomyces cerevisiae. To this end, we used a series of strains containing insertions of the reporter URA3 gene within different intergenic regions throughout four telomeres of C. glabrata. Using these reporter strains, we have assessed the roles of SIR2, SIR3, SIR4, HDF1 (yKu70), HDF2 (yKu80), and RIF1 in mediating silencing at four C. glabrata telomeres. We found that, whereas the SIR proteins are absolutely required for silencing of the reporter genes and the native subtelomeric EPA genes, the Rif1 and the Ku proteins regulate silencing at only a subset of the analyzed telomeres. We also mapped a cis element adjacent to the EPA3 locus that can silence a reporter gene when placed at a distance of 31 kb from the telomere. Our data show that silencing of the C. glabrata telomeres varies from telomere to telomere. In addition, recruitment of silencing proteins to the subtelomeres is likely, for certain telomeres, to depend both on the telomeric repeats and on particular discrete silencing elements."
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.titleyKu70/yKu80 and Rif1 regulate silencing differentially at telomeres in Candida glabrata
dc.rights.accessAcceso Abierto

Files in this item


This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional