dc.contributor.author | Gutiérrez Medina, Braulio | |
dc.contributor.author | Block, Steven M | |
dc.contributor.editor | American Association of Physics Teachers, American Institute of Physics | |
dc.date.accessioned | 2018-04-03T19:23:22Z | |
dc.date.available | 2018-04-03T19:23:22Z | |
dc.date.issued | 2010-11 | |
dc.identifier.citation | Gutierrez-Medina, B, & Block, Steven M. American Journal of Physics 78, 1152 (2010); https://doi.org/10.1119/1.3453264 | |
dc.identifier.uri | http://hdl.handle.net/11627/3787 | |
dc.description.abstract | "Microtubules are slender (similar to 25 nm diameter), filamentous polymers involved in cellular structure and organization. Individual microtubules have been visualized via fluorescence imaging of dye-labeled tubulin subunits and by video-enhanced, differential interference-contrast microscopy of unlabeled polymers using sensitive CCD cameras. We demonstrate the imaging of unstained microtubules using a microscope with conventional bright field optics in conjunction with a webcam-type camera and a light-emitting diode illuminator. The light scattered by microtubules is image-processed to remove the background, reduce noise, and enhance contrast. The setup is based on a commercial microscope with a minimal set of inexpensive components, suitable for implementation in a student laboratory. We show how this approach can be used in a demonstration motility assay, tracking the gliding motions of microtubules driven by the motor protein kinesin." | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject.classification | FÍSICA | |
dc.title | Visualizing individual microtubules by bright field microscopy | |
dc.type | article | |
dc.identifier.doi | https://doi.org/10.1119/1.3453264 | |
dc.rights.access | Acceso Abierto | |