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Optimization of human interferon gamma production in Escherichia coli by response surface methodology

dc.contributor.authorBalderas Hernández, Víctor Emmanuel
dc.contributor.authorPaz Maldonado, Luz María Teresita
dc.contributor.authorMedina Rivero, Emilio
dc.contributor.authorBarba de la Rosa, Ana Paulina
dc.contributor.authorOrdoñez Acevedo, Leandro Gabriel
dc.contributor.authorDe León Rodríguez, Antonio
dc.contributor.editorThe Korean Society for Biotechnology and Bioengineering
dc.date.accessioned2018-06-07T20:16:38Z
dc.date.available2018-06-07T20:16:38Z
dc.date.issued2008-02
dc.identifier.citationBalderas Hernández, V.E., Paz Maldonado, L.M.T., Medina Rivero, E. et al. Biotechnol Bioproc E (2008) 13: 7. https://doi.org/10.1007/s12257-007-0126-5
dc.identifier.urihttp://hdl.handle.net/11627/3843
dc.description.abstract"The production of human interferon gamma (hIFN-γ) using a synthetic gene in Escherichia coli BL21-SI was optimized by response surface methodology (RSM) and a Box-Behnken design. The process variables studied were temperature, bio-mass concentration at induction time and the NaCl concentration as inducer. According to the Box-Behnken design, a second order response function was developed. The optimal expression conditions were a temperature of 32.6°C, induction biomass of 0.31 g/L and 0.3 M NaCl in minimal medium. The model prediction for the maximum hIFN-γ production was 77.3 mg/L, which corresponded satisfactorily with the experimental data. The hIFN-γ concentration attained under optimized conditions was 13-times higher than that obtained using the non-optimized conditions. We conclude that RSM is an effective method for the optimization of recombinant protein expression using synthetic genes in E. coli."
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectEscherichia coli BL21-SI
dc.subjectMinimum medium
dc.subjectTherapeutic protein
dc.subjectsynthetic gene
dc.subject.classificationCIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA
dc.titleOptimization of human interferon gamma production in Escherichia coli by response surface methodology
dc.typearticle
dc.identifier.doihttps://doi.org/10.1007/s12257-007-0126-5
dc.rights.accessAcceso Abierto


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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