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Nitrogen sources impact hydrogen production by Escherichia coli using cheese whey as substrate

dc.contributor.authorAlvarado Cuevas, Zazil Donaxí
dc.contributor.authorOrdoñez Acevedo, Leandro Gabriel
dc.contributor.authorOrnelas Salas, José Tomás
dc.contributor.authorDe León Rodríguez, Antonio
dc.date.accessioned2018-06-07T20:16:51Z
dc.date.available2018-06-07T20:16:51Z
dc.date.issued2013-09
dc.identifier.citationZazil D. Alvarado-Cuevas, Leandro G. Ordoñez Acevedo, José Tomás Ornelas Salas, Antonio De León-Rodríguez, Nitrogen sources impact hydrogen production by Escherichia coli using cheese whey as substrate, New Biotechnology, Volume 30, Issue 6, 2013, Pages 585-590.
dc.identifier.urihttp://hdl.handle.net/11627/3870
dc.description.abstract"The impact of nitrogen source on hydrogen production by Escherichia coli WDHL (Delta hycA Delta lacI) strain using cheese whey as a substrate was evaluated. To improve the assimilation of complex proteins such as lactalbumin, we assessed treatment with a protease. Also, five external nitrogen sources were tested: NH4Cl, (NH4)(2)SO4, urea, yeast extract, and tryptone. The treatments in 120 mL serological bottles with pancreatin 1000 mg/L produced 1.75-fold more hydrogen than the cultures without pancreatin. In the bottle cultures supplemented with yeast extract or tryptone 5 g/L, hydrogen production increased up to 3.2- and 3.5-fold, respectively, whereas inorganic salts and urea had no statistical difference with respect to the control cultures. In 1-L bioreactors, the use of tryptone improved 2.1-fold hydrogen production. Tryptone or yeast extract enable the total consumption of lactose in 40 h, whereas in the control assay the lactose was not completely consumed. Our results demonstrate that it is necessary to select an adequate nitrogen source, which allows both carbon source consumption and high hydrogen production."
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBiofuels
dc.subjectHydrogen
dc.subjectCheese whey
dc.subjectNitrogen sources
dc.subject.classificationBIOLOGÍA MOLECULAR
dc.titleNitrogen sources impact hydrogen production by Escherichia coli using cheese whey as substrate
dc.typearticle
dc.identifier.doihttps://doi.org/10.1016/j.nbt.2013.03.002
dc.rights.accessAcceso Abierto


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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