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Analysis of microRNA expression in newborns with differential birth weight using newborn screening cards

dc.contributor.authorRodil García, Sandra Patricia
dc.contributor.authorArellanes Licea, Elvira del Carmen
dc.contributor.authorMontoya Contreras, Angélica
dc.contributor.authorSalazar Olivo, Luis Antonio
dc.contributor.editorMDPI AG
dc.date.accessioned2018-12-13T21:09:18Z
dc.date.available2018-12-13T21:09:18Z
dc.date.issued2017
dc.identifier.citationRodil-Garcia, P.; Arellanes-Licea, E.C.; Montoya-Contreras, A.; Salazar-Olivo, L.A. Analysis of MicroRNA Expression in Newborns with Differential Birth Weight Using Newborn Screening Cards. Int. J. Mol. Sci. 2017, 18, 2552.
dc.identifier.urihttp://hdl.handle.net/11627/4811
dc.description.abstract"Birth weight is an early predictor for metabolic diseases and microRNAs (miRNAs) are proposed as fetal programming participants. To evaluate the use of dried blood spots (DBS) on newborn screening cards (NSC) as a source of analyzable miRNAs, we optimized a commercial protocol to recover total miRNA from normal birth weight (NBW, n= 17–20), low birth weight (LBW, n = 17–20) and high birth weight (macrosomia, n = 17–20) newborns and analyzed the relative expression of selected miRNAs by stem-loop RT-qPCR. The possible role of miRNAs on the fetal programming of metabolic diseases was explored by bioinformatic tools. The optimized extraction of RNA resulted in a 1.2-fold enrichment of miRNAs respect to the commercial kit. miR-33b and miR-375 were overexpressed in macrosomia 9.8-fold (p < 0.001) and 1.7-fold, (p < 0.05), respectively and miR-454-3p was overexpressed in both LBW and macrosomia (19.7-fold, p < 0.001 and 10.8-fold, p < 0.001, respectively), as compared to NBW. Potential target genes for these miRNAs are associated to cyclic-guanosine monophosphate (cGMP)-dependent protein kinase (PKG), mitogen-activated protein kinase (MAPK), type 2 diabetes, transforming growth factor-β (TGF-β)and Forkhead box O protein (FoxO) pathways. In summary, we improved a protocol for analyzing miRNAs from NSC and provide the first evidence that birth weight modifies the expression of miRNAs associated to adult metabolic dysfunctions. Our work suggests archived NSC are an invaluable resource in the search for fetal programming biomarkers."
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCirculating microRNAs
dc.subjectNewborn screening cards
dc.subjectBirth weight
dc.subjectFetal programming
dc.subject.classificationMEDICINA Y CIENCIAS DE LA SALUD
dc.titleAnalysis of microRNA expression in newborns with differential birth weight using newborn screening cards
dc.typearticle
dc.identifier.doihttps://doi.org/10.3390/ijms18122552
dc.rights.accessAcceso Abierto


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional