Mostrar el registro sencillo del ítem

Título

Evaluation of the expression and function of the P2X7 receptor and ART1 in human regulatory T-cell subsets

dc.contributor.authorCortés García, Juan Diego
dc.contributor.authorLópez López, Cintya del Refugio
dc.contributor.authorCortez Espinosa, Nancy
dc.contributor.authorGarcía Hernández, Mariana Haydee
dc.contributor.authorGuzman-Flores, Juan Manuel
dc.contributor.authorLayseca Espinosa, Esther
dc.contributor.authorPortales Cervantes, Liliana
dc.contributor.authorPortales Pérez, Diana Patricia
dc.date.accessioned2019-08-09T22:22:40Z
dc.date.available2019-08-09T22:22:40Z
dc.date.issued2016
dc.identifier.citationJuan D. Cortés-Garcia, Cintya López-López, Nancy Cortez-Espinosa, Mariana H. García-Hernández, Juan M. Guzmán-Flores, Esther Layseca-Espinosa, Liliana Portales-Cervantes, Diana P. Portales-Pérez, Evaluation of the expression and function of the P2X7 receptor and ART1 in human regulatory T-cell subsets, Immunobiology, Volume 221, Issue 1, 2016, Pages 84-93.
dc.identifier.urihttp://hdl.handle.net/11627/5026
dc.description.abstract"Regulatory T cells that express CD39 (CD39+ Treg) exhibit specific immunomodulatory properties. Ectonucleotidase CD39 hydrolyses ATP and ADP. ATP is a ligand of the P2X7 receptor and induces the shedding of CD62L and apoptosis. However, the role of ATP in CD39+ Treg cells has not been defined. Furthermore, NAD can activate the P2X7 receptor via ADP-ribosyltransferase (ART) enzymes and cause cell depletion in murine models. We evaluated the expression and function of P2X7 and ART1 in CD39+ Treg and CD39- Treg cells in the presence or absence of ATP and NAD. We isolated peripheral blood mononuclear cells from healthy subjects and purified CD4+ T cells, CD4+ CD25+ T cells and CD4+ CD25+ CD39+ T cells. P2X7 and ART1 expression was assessed by flow cytometry and real-time PCR. Our results showed low P2X7 expression on CD39+ Treg cells and higher levels of ART1 expression in CD4+ CD39+ T cells than the other subtypes studied. Neither shedding of CD62L nor cell death of CD39+ Treg or CD39- Treg cells was observed by 1mM ATP or 60 mu M NAD. In contrast, P2Xs receptor-dependent proliferation with 300 p,M ATP, was inhibited by NAD in the different cell types analysed. The NAD proliferation-inhibition was increased with P2X5 and A2a agonist and was reversed with P2X5 and A2a antagonist, therefore NAD inhibits P2Xs-dependent proliferation and A2a activation. In conclusion, our results suggest that the altered function and expression of P2X7 and ART1 in the human CD39+ Treg or CD39 Treg cells could participate in the resistance against cell death induced by ATP or NAD. (C) 2015 Elsevier GmbH. All rights reserved."
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectP2X7 receptor
dc.subjectART1
dc.subjectATP
dc.subjectNAD
dc.subjectImmune regulation
dc.subjectCD39
dc.subject.classificationBIOLOGÍA MOLECULAR
dc.titleEvaluation of the expression and function of the P2X7 receptor and ART1 in human regulatory T-cell subsets
dc.typearticle
dc.identifier.doihttps://doi.org/10.1016/j.imbio.2015.07.018
dc.rights.accessAcceso Abierto


Ficheros en el ítem

Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Excepto si se señala otra cosa, la licencia del ítem se describe como Attribution-NonCommercial-NoDerivatives 4.0 Internacional