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Silencing of OCH1 unveils the role of Sporothrix schenckii N-linked glycans during the host-fungus interaction

dc.contributor.authorLozoya Pérez, Nancy Edith
dc.contributor.authorCasas Flores, J. Sergio
dc.contributor.authorFogaca de Almeida, José Roberto
dc.contributor.authorMartínez Álvarez, José Ascención
dc.contributor.authorLópez-Ramírez, Luz Adriana
dc.contributor.authorJannuzzi, Grasielle
dc.contributor.authorTrujillo Esquivel, José Elías
dc.contributor.authorEstrada Mata, Eine
dc.contributor.authorAlmeida, Sandro R.
dc.contributor.authorFranco, Bernardo
dc.contributor.authorLopes Bezerra, Leila María
dc.contributor.authorMora Montes, Héctor Manuel
dc.identifier.citationLozoya-Pérez NE, Casas-Flores S, de Almeida JR, Martínez-Álvarez JA, López-Ramírez LA, Jannuzzi GP, Trujillo-Esquivel E, Estrada-Mata E, Almeida SR, Franco B, Lopes-Bezerra LM, Mora-Montes HM. Silencing of OCH1 unveils the role of Sporothrix schenckii N-linked glycans during the host fungus interaction. Infect Drug Resist. 2019;12:67-85
dc.description.abstract"Background: Sporothrix schenckii is a neglected fungal pathogen for the human being and other mammals. In several fungal systems, Och1 is a Golgi alpha 1,6-mannosyltransferase with a key function in the synthesis of N-linked glycans; which are important elements during the host-fungus interplay. The role of OCH1 in fungal virulence seems to be species-specific, being an essential component for Candida albicans virulence and dispensable during the interaction of Aspergillus fumigatus with the host. Methods: Here, we silenced S. schenckii OCH1 and characterized the phenotype of the mutant strains. Results: The mutant strains did not show defects in the cell or colony morphology, the growth rate or the ability to undergo dimorphism; but the cell wall changed in both composition and exposure of inner components at the surface. When interacting with human monocytes, the silenced strains had a reduced ability to stimulate TNF alpha and IL-6 but stimulated higher levels of IL-10. The interaction with hitman macrophages was also altered, with reduced numbers of silenced cells phagocytosed. These strains showed virulence attenuation in both Galleria mellonella and in the mouse model of sporotrichosis. Nonetheless, the cytokine levels in infected organs did not vary significantly when compared with the wild-type strain. Conclusion: Our data demonstrate that OCH1 silencing affects different aspects of the S. schenckii-host interaction."
dc.publisherDove Press Ltd
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.subjectCell wall
dc.subjectProtein glycosylation
dc.subjectGene silencing
dc.subjectHost-fungus interplay
dc.subject.classificationENFERMEDADES INFECCIOSAS
dc.titleSilencing of OCH1 unveils the role of Sporothrix schenckii N-linked glycans during the host-fungus interaction
dc.rights.accessAcceso Abierto

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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