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Toxicological Screening of Four Bioactive Citroflavonoids: In Vitro, In Vivo, and In Silico Approaches

dc.contributor.authorOrtiz Andrade, Rolffy Ruben
dc.contributor.authorAraujo León, Jesús Alfredo
dc.contributor.authorSánchez Recillas, Amanda
dc.contributor.authorNavarrete Vázquez, Juan Gabriel
dc.contributor.authorGonzález Sánchez, Avel Adolfo
dc.contributor.authorHidalgo Figueroa, Sergio Nemorio
dc.contributor.authorAlonso Castro, Angel Josabab
dc.contributor.authorAranda González, Irma
dc.contributor.authorHernández Núñez, Emanuel
dc.contributor.authorCoral Martínez, Tania Isolina
dc.contributor.authorSánchez Salgado, Juan Carlos
dc.contributor.authorYáñez Pérez, Victor
dc.contributor.authorLucio Garcia, Monica Arely
dc.identifier.citationOrtiz-Andrade, R.; Araujo-León, J.A.; Sánchez-Recillas, A.; Navarrete-Vazquez, G.; González-Sánchez, A.A.; Hidalgo-Figueroa, S.; Alonso-Castro, Á.J.; Aranda-González, I.; Hernández-Núñez, E.; Coral-Martínez, T.I.; Sánchez-Salgado, J.C.; Yáñez-Pérez, V.; Lucio-Garcia, M.A. Toxicological Screening of Four Bioactive Citroflavonoids: In Vitro, In Vivo, and In Silico Approaches. Molecules 2020, 25, 5959.
dc.description.abstract"Many studies describe different pharmacological effects of flavonoids on experimental animals and humans. Nevertheless, few ones are confirming the safety of these compounds for therapeutic purposes. This study aimed to investigate the preclinical safety of naringenin, naringin, hesperidin, and quercetin by in vivo, in vitro, and in silico approaches. For this, an MTT-based cytotoxicity assay in VERO and MDCK cell lines was performed. In addition, acute toxicity was evaluated on Wistar rats by OECD Guidelines for the Testing of Chemicals (Test No. 423: Acute Oral Toxicity-Class Method). Furthermore, we used the ACD/Tox Suite to predict toxicological parameters such as hERG channel blockade, CYP450 inhibition, and acute toxicity in animals. The results showed that quercetin was slightly more cytotoxic on cell lines (IC50 of 219.44 ± 7.22 mM and 465.41 ± 7.44 mM, respectively) than the other citroflavonoids. All flavonoids exhibited an LD50 value > 2000 mg/kg, which classifies them as low-risk substances as OECD guidelines established. Similarly, predicted LD50 was LD50 > 300 to 2000 mg/kg for all flavonoids as acute toxicity assay estimated. Data suggests that all these flavonoids did not show significant toxicological effects, and they were classified as low-risk, useful substances for drug development."
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.subjectLow-risk substances
dc.subjectAcute oral toxicity
dc.subjectMTT-based assay
dc.subjectToxicity prediction
dc.titleToxicological Screening of Four Bioactive Citroflavonoids: In Vitro, In Vivo, and In Silico Approaches
dc.rights.accessAcceso Abierto

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