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Diisononyl Phthalate differentially affects sirtuin expression in the HepG2 cell line

dc.contributor.authorGutiérrez García, Ana Karen
dc.contributor.authorChoudhury, Mahua
dc.contributor.authorDe León Rodríguez, Antonio
dc.identifier.citationGutiérrez-García AK, Choudhury M, De Leon-Rodriguez A. Diisononyl Phthalate Differentially Affects Sirtuin Expression in the HepG2 Cell Line. Chem Res Toxicol. 2019 Sep 16;32(9):1863-1870. doi: 10.1021/acs.chemrestox.9b00206. Epub 2019 Aug 27. PMID: 31423773.
dc.description.abstract"Human exposure to phthalates has received special attention due to their possible adverse human health effects. Diisononyl phthalate (DINP) is a plasticizer still widely used in many products, despite being considered an endocrine disruptor. In this study, we evaluated DINP’s cytotoxicity, its effect on the levels of reactive oxygen species (ROS), and its effect on sirtuin expression in HepG2 cells. Results showed that 1 ?g/mL DINP significantly downregulated Sirt1, Sirt2, Sirt3, and Sirt5 gene expression (p < 0.05), while other sirtuins remained unaffected. Furthermore, protein levels of Sirt1 and Sirt3 were significantly downregulated by 1 ?g/mL DINP. On the other hand, 100 ?g/mL DINP doubled the levels of lysine acetylation proteins (increased 2-fold) as well as reactive oxygen species (ROS) compared with the controls. In conclusion, our study suggests, for the first time, that DINP regulates the potential epigenetic disruptor sirtuin family and leads to induction of ROS via sirtuins."
dc.publisherAmerican Chemical Society
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.subjectPeptides and proteins
dc.subjectFree radicals
dc.subjectOrganic compounds
dc.subjectOxidative stress
dc.titleDiisononyl Phthalate differentially affects sirtuin expression in the HepG2 cell line
dc.rights.accessAcceso Abierto

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional