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Expression and characterization of a recombinant psychrophilic Cu/Zn superoxide dismutase from deschampsia antarctica E. Desv. [Poaceae]
(2015-04)
"We present here the structural modeling and biochemical characterization of a recombinant superoxide dismutase (SOD) from Deschampsia antarctica E. Desv. [Poaceae] produced in Escherichia coli. The recombinant protein was ...
Optimization of human interferon gamma production in Escherichia coli by response surface methodology
(2008-02)
"The production of human interferon gamma (hIFN-γ) using a synthetic gene in Escherichia coli BL21-SI was optimized by response surface methodology (RSM) and a Box-Behnken design. The process variables studied were ...
Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-γ but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli
(2007-09)
"Production of periplasmic human interferon-γ (hINF-γ) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which ...
Optimization of fermentation conditions for the production of the mezcal from Agave salmiana using response surface methodology
(Elsevier, 2008-01)
"Response surface methodology was applied to optimize the fermentative phase for the mezcal production from Agave salmiana. A 3k factorial design was used to obtain models describing the relationship between the ethanol ...
Identification of calcium stress induced genes in amaranth leaves through suppression subtractive hybridization
(Elsevier, 2011-11)
"Calcium (Ca2+) is a critical ion for the growth and development of plants and plays an important role in signal transduction pathways in response to biotic and abiotic stresses. We investigated the Ca2+ stress responsive-genes ...
Identification of yeast and bacteria involved in the mezcal fermentation of Agave salmiana
(Wiley, 2008-06)
"Aims: To identify the yeast and bacteria present in the mezcal fermentation from Agave salmiana. Methods and Results: The restriction and sequence analysis of the amplified region, between 18S and 28S rDNA and 16S rDNA ...
Expansión de células CD34+ de sangre de cordón umbilical en frascos giratoriosExpansion of CD34+ human hematopoietic cells from umbilical cord blood using roller bottles
(2014-08)
"El transplante de células madre hematopoyéticas está limitado por la cantidad de células CD34+ presentes en las unidades de sangre de cordón umbilical; el objetivo de este trabajo fue obtener la expansión in vitro de ...
Expansion of human hematopoietic cells from umbilical cord blood using roller bottles in CO2 and CO2-free atmosphere
(2011-04)
"In this work, we evaluated the expansion of human hematopoietic stem cells from umbilical cord blood in roller bottles. The Iscove's modified Dulbecco's medium, the Stem Pro 34-SFM medium, and the L-15 Leibovitz's medium ...
Identification of differential expressed transcripts in cervical cancer of Mexican patients
(2011-06)
"The aim of this study was to identify the gene expression profile in biopsies of patients with cervical intraepithelial neoplasia (CIN) 1, CIN 2, CIN 3, and microinvasive cancer by suppression subtractive hybridization ...
HLA-C genotype and TCR vβ expression analysis in Mexican patients with Psoriasis
(2011)
"Genetic background and T-cell expansion have been associated as the most important factors for psoriasis susceptibility in the Caucasian population. This study was performed to identify the T cell receptor Vβ repertoire ...