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Expression of EPL1 from Trichoderma atroviride in Arabidopsis Confers Resistance to Bacterial and Fungal Pathogens

dc.contributor.authorRojas Moreno, Monica Montserrat
dc.contributor.authorGonzález Pérez, Enrique
dc.contributor.authorRodríguez Hernández, Aída Araceli
dc.contributor.authorOrtega Amaro, María Azucena
dc.contributor.authorBecerra Flora, Alicia
dc.contributor.authorSerrano, Mario
dc.contributor.authorJiménez Bremont, Juan Francisco
dc.date.accessioned2024-05-30T21:28:39Z
dc.date.available2024-05-30T21:28:39Z
dc.date.issued2023
dc.identifier.citationRojas Moreno, M.M.; González-Pérez, E.; Rodríguez-Hernandez, A.A.; Ortega-Amaro, M.A.; Becerra-Flora, A.; Serrano, M.; Jiménez-Bremont, J.F. Expression of EPL1 from Trichoderma atroviride in Arabidopsis Confers Resistance to Bacterial and Fungal Pathogens. Plants 2023, 12, 2443. https://doi.org/10.3390/plants12132443
dc.identifier.urihttp://hdl.handle.net/11627/6578
dc.description.abstractDuring plant interaction with beneficial microorganisms, fungi secrete a battery of elicitors that trigger plant defenses against pathogenic microorganisms. Among the elicitor molecules secreted by Trichoderma are cerato-platanin proteins, such as EPL1, from Trichoderma atroviride. In this study, Arabidopsis thaliana plants that express the TaEPL1 gene were challenged with phytopathogens to evaluate whether expression of EPL1 confers increased resistance to the bacterial pathogen Pseudomonas syringae and the necrotrophic fungus Botrytis cinerea. Infection assays showed that Arabidopsis EPL1-2, EPL1-3, EPL1-4 expressing lines were more resistant to both pathogens in comparison to WT plants. After Pseudomonas syringae infection, there were reduced disease symptoms (e.g., small chlorotic spots) and low bacterial titers in the three 35S::TaEPL1 expression lines. Similarly; 35S::TaEPL1 expression lines were more resistant to Botrytis cinerea infection, showing smaller lesion size in comparison to WT. Interestingly, an increase in ROS levels was detected in 35S::TaEPL1 expression lines when compared to WT. A higher expression of SA- and JA-response genes occurred in the 35S::TaEPL1 lines, which could explain the resistance of these EPL1 expression lines to both pathogens. We propose that EPL1 is an excellent elicitor, which can be used to generate crops with improved resistance to broad-spectrum diseases.
dc.publisherMDPI
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectArabidopsis thaliana
dc.subjectBotrytis cinerea
dc.subjectCerato-platanin proteins
dc.subjectElicitor
dc.subjectPseudomonas syringae
dc.subjectTrichoderma atroviride
dc.subject.classificationBIOLOGÍA VEGETAL (BOTÁNICA)
dc.titleExpression of EPL1 from Trichoderma atroviride in Arabidopsis Confers Resistance to Bacterial and Fungal Pathogens
dc.typearticle
dc.identifier.doihttps://doi.org/10.3390/plants12132443
dc.rights.accessAcceso Abierto


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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