Show simple item record

Title

Neo-functionalization in Saccharomyces cerevisiae: a novel Nrg1-Rtg3 chimeric transcriptional modulator is essential to maintain mitochondrial DNA integrity

dc.contributor.authorCampero Basaldúa, José Carlos
dc.contributor.authorGonzález Flores, James
dc.contributor.authorGarcía, Janeth Alejandra
dc.contributor.authorRamírez González, Edgar Adrián
dc.contributor.authorHernández, Hugo
dc.contributor.authorAguirre, Beatriz
dc.contributor.authorTorres Ramírez, Nayeli
dc.contributor.authorMárquez, Dariel
dc.contributor.authorSánchez, Norma Silvia
dc.contributor.authorGómez Hernández, Nicolás
dc.contributor.authorTorres Machorro, Ana Lilia
dc.contributor.authorRiego Ruíz, Lina Raquel
dc.contributor.authorScazzocchio, Claudio
dc.contributor.authorGonzález Manjarrez, Alicia
dc.date.accessioned2024-05-30T21:28:43Z
dc.date.available2024-05-30T21:28:43Z
dc.date.issued2023
dc.identifier.citationCampero-Basaldua Carlos, González James, García Janeth Alejandra, Ramírez Edgar, Hernández Hugo, Aguirre Beatriz, Torres-Ramírez Nayeli, Márquez Dariel, Sánchez Norma Silvia, Gómez-Hernández Nicolás, Torres-Machorro Ana Lilia, Riego-Ruiz Lina, Scazzocchio Claudio and González Alicia 2023Neo-functionalization in Saccharomyces cerevisiae: a novel Nrg1-Rtg3 chimeric transcriptional modulator is essential to maintain mitochondrial DNA integrityR. Soc. Open Sci.10231209
dc.identifier.urihttp://hdl.handle.net/11627/6584
dc.description.abstractIn Saccharomyces cerevisiae, the transcriptional repressor Nrg1 (Negative Regulator of Glucose-repressed genes) and the ?-Zip transcription factor Rtg3 (ReTroGrade regulation) mediate glucose repression and signalling from the mitochondria to the nucleus, respectively. Here, we show a novel function of these two proteins, in which alanine promotes the formation of a chimeric Nrg1/Rtg3 regulator that represses the ALT2 gene (encoding an alanine transaminase paralog of unknown function). An NRG1/NRG2 paralogous pair, resulting from a post-wide genome small-scale duplication event, is present in the Saccharomyces genus. Neo-functionalization of only one paralog resulted in the ability of Nrg1 to interact with Rtg3. Both nrg1? and rtg3? single mutant strains were unable to use ethanol and showed a typical petite (small) phenotype on glucose. Neither of the wild-type genes complemented the petite phenotype, suggesting irreversible mitochondrial DNA damage in these mutants. Neither nrg1? nor rtg3? mutant strains expressed genes encoded by any of the five polycistronic units transcribed from mitochondrial DNA in S. cerevisiae. This, and the direct measurement of the mitochondrial DNA gene complement, confirmed that irreversible damage of the mitochondrial DNA occurred in both mutant strains, which is consistent with the essential role of the chimeric Nrg1/Rtg3 regulator in mitochondrial DNA maintenance.
dc.publisherThe Royal Society
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectTranscriptional coregulators
dc.subjectRespiratory metabolism
dc.subjectMitochondrial genes
dc.subjectNeo-functionalization
dc.subject.classificationCIENCIAS TECNOLÓGICAS
dc.titleNeo-functionalization in Saccharomyces cerevisiae: a novel Nrg1-Rtg3 chimeric transcriptional modulator is essential to maintain mitochondrial DNA integrity
dc.typearticle
dc.identifier.doihttps://doi.org/10.1098/rsos.231209
dc.rights.accessAcceso Abierto


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional