Show simple item record

Title

Incomptine A Induces Apoptosis, ROS Production and a Differential Protein Expression on Non-Hodgkin’s Lymphoma Cells

dc.contributor.authorPina Jiménez, Emmanuel
dc.contributor.authorCalzada, Fernando
dc.contributor.authorBautista Redonda, Francisco Elihú
dc.contributor.authorOrdoñez Razo, Rosa María
dc.contributor.authorVelázquez, Claudia
dc.contributor.authorBarbosa Cabrera, Reyna Elizabeth
dc.contributor.authorGarcía Hernández, Normand
dc.date.accessioned2022-03-10T19:33:37Z
dc.date.available2022-03-10T19:33:37Z
dc.date.issued2021
dc.identifier.citationPina-Jiménez, E.; Calzada, F.; Bautista, E.; Ordoñez-Razo, R.M.; Velázquez, C.; Barbosa, E.; García-Hernández, N. Incomptine A Induces Apoptosis, ROS Production and a Differential Protein Expression on Non-Hodgkin’s Lymphoma Cells. Int. J. Mol. Sci. 2021, 22, 10516. https://doi.org/10.3390/ijms221910516
dc.identifier.urihttp://hdl.handle.net/11627/5761
dc.description.abstract"Sesquiterpene lactones are of pharmaceutical interest due their cytotoxic and antitumor properties, which are commonly found within plants of several genera from the Asteraceae family such as the Decachaeta genus. From Decachaeta incompta four heliangolide, namely incomptines A-D have been isolated. In this study, cytotoxic properties of incomptine A (IA) were evaluated on four lymphoma cancer cell lines: U-937, Farage, SU-DHL-2, and REC-1. The type of cell death induced by IA and its effects on U-937 cells were analyzed based on its capability to induce apoptosis and produce reactive oxygen species (ROS) through flow cytometry with 4?,6-diamidino-2-phenylindole staining, dual annexin V/DAPI staining, and dichlorofluorescein 2?,7?-diacetate, respectively. A differential protein expression analysis study was carried out by isobaric tags for relative and absolute quantitation (iTRAQ) through UPLC-MS/MS. Results reveal that IA exhibited cytotoxic activity against the cell line U-937 (CC50 of 0.12 ± 0.02 ?M) and the incubation of these cells in presence of IA significantly increased apoptotic population and intracellular ROS levels. In the proteomic approach 1548 proteins were differentially expressed, out of which 587 exhibited a fold-change ? 1.5 and 961 a fold-change ? 0.67. Most of these differentially regulated proteins are involved in apoptosis, oxidative stress, glycolytic metabolism, or cytoskeleton structuration."
dc.publisherMDPI
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectincomptine A
dc.subjectSesquiterpene lactone
dc.subjectDecachaeta incompta
dc.subjectCytotoxic activity
dc.subjectiTRAQ
dc.subjectApoptosis
dc.subjectROS production
dc.subject.classificationBIOLOGÍA MOLECULAR
dc.titleIncomptine A Induces Apoptosis, ROS Production and a Differential Protein Expression on Non-Hodgkin’s Lymphoma Cells
dc.typearticle
dc.identifier.doihttps://doi.org/10.3390/ijms221910516
dc.rights.accessAcceso Abierto


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional