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Optical sectioning of unlabeled samples using bright-field microscopy

dc.contributor.authorGutiérrez Medina, Braulio
dc.date.accessioned2023-06-14T16:12:32Z
dc.date.available2023-06-14T16:12:32Z
dc.date.issued2022
dc.identifier.citationGutiérrez-Medina, Braulio. (2022). Optical sectioning of unlabeled samples using bright-field microscopy. Proceedings of the National Academy of Sciences. 119. DOI: 10.1073/pnas.2122937119.
dc.identifier.urihttp://hdl.handle.net/11627/6359
dc.description.abstract"The bright-field (BF) optical microscope is a traditional bioimaging tool that has been recently tested for depth discrimination during evaluation of specimen morphology; however, existing approaches require dedicated instrumentation or extensive computer modeling. We report a direct method for three-dimensional (3D) imaging in BF microscopy, applicable to label-free samples, where we use Köhler illumination in the coherent regime and conventional digital image processing filters to achieve optical sectioning. By visualizing fungal, animal tissue, and plant samples and comparing with light-sheet fluorescence microscopy imaging, we demonstrate the accuracy and applicability of the method, showing how the standard microscope is an effective 3D imaging device."
dc.publisherNational Academy of Science
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectThree-dimensional microscopy
dc.subjectOptical sectioning
dc.subjectBright field
dc.subjectLabel-free imaging
dc.subjectImage processing
dc.subject.classificationCIENCIAS TECNOLÓGICAS
dc.titleOptical sectioning of unlabeled samples using bright-field microscopy
dc.typearticle
dc.identifier.doihttps://doi.org/10.1073/pnas.2122937119
dc.rights.accessAcceso Abierto


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional