Browsing División de Biología Molecular by Author "De León Rodríguez, Antonio"
Now showing items 41-60 of 87
-
Expansion of hematopoietic stem cells from umbilical cord blood in roller bottles.
Andrade Zaldívar, Hera (2010)"Los transplantes de progenitores hematopoyéticos de sangre de cordón umbilical son empleados en una variedad de padecimientos hematológicos y oncológicos cada vez con más frecuencia. En la mayoría de los casos, el número ... -
Expansion of human hematopoietic cells from umbilical cord blood using roller bottles in CO2 and CO2-free atmosphere
Andrade Zaldívar, Hera; Kalixto Sánchez, Marco Antonio; Barba de la Rosa, Ana Paulina; De León Rodríguez, Antonio (2011-04)"In this work, we evaluated the expansion of human hematopoietic stem cells from umbilical cord blood in roller bottles. The Iscove's modified Dulbecco's medium, the Stem Pro 34-SFM medium, and the L-15 Leibovitz's medium ... -
Expansion of human hematopoietic stem cells for transplantation: trends and perspectives
Zaldivar Andrade, Hera; Santos Martínez, Martha Leticia; De León Rodríguez, Antonio (2008-03)"Umbilical cord blood transplantation is clinically limited by its low progenitor cell content. Ex vivo expansion has become an alternative to increase the cell dose available for transplants. Expansion has been evaluated ... -
Expansión de células CD34+ de sangre de cordón umbilical en frascos giratorios
Andrade Zaldívar, Hera; Barba de la Rosa, Ana Paulina; De León Rodríguez, Antonio (2014-08)"El transplante de células madre hematopoyéticas está limitado por la cantidad de células CD34+ presentes en las unidades de sangre de cordón umbilical; el objetivo de este trabajo fue obtener la expansión in vitro de ... -
Expresión de interferón gamma humano y la proteína verde fluorescente en E. coli usando el sistema de autotransporte ShdA.
Solís Andrade, Karla Ivon (2012)"La exportación de proteínas recombinantes en Escherichia coli, ya sea ancladas o liberadas fuera de la membrana externa es de gran utilidad en muchas aplicaciones biotecnológicas. En bacterias Gram–negativas se conocen ... -
Expresión de interleucina -10 humana recombinante en Bifidobacterium longum y Escherichia coli.
Reyes Escogido, María de Lourdes (2006)"Bifidobacterium longum es una bacteria Gram positiva no patógena que forma parte de un grupo de microorganismos conocidos como probióticos. Recientemente, el interés en esta bacteria ha aumentado considerablemente no solo ... -
Expresión de una proteína de fusión vasostatina 30 y vasoinhibina 14.1 (Osiris) en Pichia pastoris y evaluación de la actividad biológica de la Vasostatina 30 en pez cebra.
Juvera Avalos, Edgar Rodrigo (2019-08-10)"El proceso angiogénico consiste en la formación de nuevos vasos sanguíneos a partir de los preexistentes y son clave en el crecimiento y metástasis del cáncer; estos proveen oxígeno y nutrientes que permiten el crecimiento ... -
Expresión heteróloga de péptidos antiangiogénicos en Pichia pastoris
Calderón Salais, Sergio (2018-09)"La vasostatina (Vs30) y la vasoinhibina (Vi-II-14.1) son péptidos inhibidores de la angiogénesis que son derivados de la región N-terminal de la calreticulina y la prolactina humana, respectivamente. Anteriormente, Vs30, ... -
Expression and characterization of a recombinant psychrophilic Cu/Zn superoxide dismutase from deschampsia antarctica E. Desv. [Poaceae]
Rojas Contreras, Juan Antonio; Barba de la Rosa, Ana Paulina; De León Rodríguez, Antonio (2015-04)"We present here the structural modeling and biochemical characterization of a recombinant superoxide dismutase (SOD) from Deschampsia antarctica E. Desv. [Poaceae] produced in Escherichia coli. The recombinant protein was ... -
Fermentation of lactose and its constituent sugars by Escherichia coli WDHL: impact on hydrogen production
Rosales Colunga, Luis Manuel; Razo Flores, Elías; De León Rodríguez, Antonio (Elsevier, 2012-05)"Fermentations of lactose, glucose and galactose using Escherichia coli WDHL, a hydrogen over producer strain, were performed. With glucose as substrate pyruvate was mainly routed to the lactate pathway, resulting in ... -
Fermentative biohydrogen production: trends and perspectives
Dávila Vázquez, Gustavo; Arriaga García, Sonia Lorena; Alatriste Mondragón, Felipe; De León Rodríguez, Antonio; Rosales Colunga, Luis Manuel; Razo Flores, Elías (2008-01)"Biologically produced hydrogen (biohydrogen) is a valuable gas that is seen as a future energy carrier, since its utilization via combustion or fuel cells produces pure water. Heterotrophic fermentations for biohydrogen ... -
Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica
García Echauri, Sergio Ángel; Ordoñez Acevedo, Leandro Gabriel; Rojas Contreras, Juan Antonio; Barba de la Rosa, Ana Paulina; De León Rodríguez, Antonio (Elsevier, 2009-09)"Superoxide dismutase (SOD) catalyzes the conversion of the superoxide radical (O2−) into oxygen and hydrogen peroxide. Deschampsia antarctica is a plant that grows in Antarctica and survives to extreme low temperature and ... -
Heterologous expression of Trichoderma atroviride endochitinase ech42 in Pichia pastoris at low and high dissolved oxygen tensions
Pérez Martínez, Ana Silvia; Barba de la Rosa, Ana Paulina; De León Rodríguez, Antonio (2014-04)"The expression of recombinant Trichoderma atroviride endochitinase ech42 (rech42) in Pichia pastoris driven by the constitutive PGAP promoter was tested at low (3%) and high (40%) dissolved oxygen tension (DOT) conditions. ... -
HLA-C genotype and TCR vβ expression analysis in Mexican patients with Psoriasis
Santos Martínez, Martha Leticia; León Galvan, Ma Fabiola; Marino Marmolejo, Erika Nahomy; De León Rodríguez, Antonio; Barba de la Rosa, Ana Paulina; Moncada González, Benjamín (2011)"Genetic background and T-cell expansion have been associated as the most important factors for psoriasis susceptibility in the Caucasian population. This study was performed to identify the T cell receptor Vβ repertoire ... -
Hydrogen production by Escherichia coli genetically modified
Rosales Colunga, Luis Manuel (2011-10)"Due to its high energy content and because its use only result in water as subproduct, hydrogen is seen as the energetic carrier for the future. Among the methods of production of this gas, the biological processes are ... -
Hydrogen production by Escherichia coli ΔhycA ΔlacI using cheese whey as substrate
Rosales Colunga, Luis Manuel; Razo Flores, Elías; Ordoñez Acevedo, Leandro Gabriel; Alatriste Mondragón, Felipe; De León Rodríguez, Antonio (Elsevier, 2010-01)"This study reports a fermentative hydrogen production by Escherichia coli using cheese whey as substrate. To improve the biohydrogen production, an E. coli ΔhycA ΔlacI strain (WDHL) was constructed. The absence of hycA ... -
Identification of calcium stress induced genes in amaranth leaves through suppression subtractive hybridization
Aguilar Hernández, Hugo Sergio; Santos Martínez, Martha Leticia; León Galvan, Ma Fabiola; Barrera Pacheco, Alberto; Espitia Rangel, Eduardo; De León Rodríguez, Antonio; Guevara González, Ramón Gerardo; Barba de la Rosa, Ana Paulina (Elsevier, 2011-11)"Calcium (Ca2+) is a critical ion for the growth and development of plants and plays an important role in signal transduction pathways in response to biotic and abiotic stresses. We investigated the Ca2+ stress responsive-genes ... -
Identification of differential expressed transcripts in cervical cancer of Mexican patients
Santos Martínez, Martha Leticia; León Galvan, Ma Fabiola; Marino Marmolejo, Erika Nahomy; Barba de la Rosa, Ana Paulina; De León Rodríguez, Antonio; González Amaro, Roberto; Guevara González, Ramón Gerardo (2011-06)"The aim of this study was to identify the gene expression profile in biopsies of patients with cervical intraepithelial neoplasia (CIN) 1, CIN 2, CIN 3, and microinvasive cancer by suppression subtractive hybridization ... -
Identification of yeast and bacteria involved in the mezcal fermentation of Agave salmiana
Escalante Minakata, María del Pilar; Blaschek, Hans Peter M.; Barba de la Rosa, Ana Paulina; Santos Martínez, Martha Leticia; De León Rodríguez, Antonio (Wiley, 2008-06)"Aims: To identify the yeast and bacteria present in the mezcal fermentation from Agave salmiana. Methods and Results: The restriction and sequence analysis of the amplified region, between 18S and 28S rDNA and 16S rDNA ... -
Indole modifies the central carbon flux in the anaerobic metabolism of Escherichia coli: application to the production of hydrogen and other metabolites
De León Rodríguez, Antonio; Caño Muñiza, Santiago L; Liu, Junyang; Summers, David K (Elsevier, 2016-12)"Indole is a bicyclic signaling molecule with effects on both eukaryotic and prokaryotic cells. The majority of studies of indole action have been performed with bacteria cultured under aerobic conditions and little ...