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Molecular and biochemical characterization of extracellular tannin acyl hydrolase activity from a Mexican isolate of Aspergillus niger

dc.contributor.authorLeón Galvan, Ma Fabiola
dc.contributor.authorTorres Pacheco, Irineo
dc.contributor.authorGuevara Olvera, Lorenzo
dc.contributor.authorBarba de la Rosa, Ana Paulina
dc.contributor.authorGonzález Chavira, Mario Martín
dc.contributor.authorGuevara González, Ramón Gerardo
dc.contributor.editorNational Institute of Science Communication And Information Resources
dc.date.accessioned2018-03-23T23:59:41Z
dc.date.available2018-03-23T23:59:41Z
dc.date.issued2010-12
dc.identifier.urihttp://hdl.handle.net/11627/3706
dc.description.abstract"Microbial tannase, a hydrolysable tannin-degrading enzyme, is extensively used in manufacture of instant tea, beer, wine, and gallic acid. Aspergillus niger strain, obtained from a Mexican tannery wastewaters rich in gallic acid [Quebracho Phenolics-rich Tannery Wastewaters, (QPTW)], displayed a good growth and tannase activity in a minimal medium added with 1% (w/v) QPTW (Kr= 0.451 mm.h-1). Using PCR and RACE 3´ and 5´methodologies, a complete cDNA of a tannase was cloned from this isolate.Nucleotide sequence of complete cDNA was of 4690 bp with a complete ORF of 1833 bp encoding 611 amino acids. Transcriptionalinduction was observed in mineral medium added with carbon sources as tannic acid alone (1 and 10 g/l), as well as mix of glucose(1 and 10 g/l) and tannic acid (1 g/l) in the media. However, neither glucose (1 and 10 g/l) and sucrose (1 and 10 g/l) nor (+)-catechin(1 and 10 g/l) as sole carbon sources displayed gene induction in in vitro assays. A. niger-GTO is a new strain with interesting characteristics for industrial tannase production purposes."
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectTannase
dc.subjectAspergillus niger
dc.subjectGuanajuato isolate
dc.subjectcDNA cloning
dc.subject.classificationINGENIERÍA Y TECNOLOGÍA
dc.titleMolecular and biochemical characterization of extracellular tannin acyl hydrolase activity from a Mexican isolate of Aspergillus niger
dc.typearticle
dc.rights.accessAcceso Abierto


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional