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Ultrastructural analysis of Candida albicans when exposed to silver nanoparticles

dc.contributor.authorVázquez Muñoz, Roberto
dc.contributor.authorÁvalos Borja, Miguel
dc.contributor.authorCastro Longoria, Ernestina
dc.contributor.editorPublic Library of Science
dc.date.accessioned2018-04-03T19:23:27Z
dc.date.available2018-04-03T19:23:27Z
dc.date.issued2014-10
dc.identifier.citationVazquez-Muñoz R, Avalos-Borja M, Castro-Longoria E (2014) Ultrastructural Analysis of Candida albicans When Exposed to Silver Nanoparticles. PLoS ONE 9(10): e108876. https://doi.org/10.1371/journal.pone.0108876
dc.identifier.urihttp://hdl.handle.net/11627/3807
dc.description.abstract"Candida albicans is the most common fungal pathogen in humans, and recently some studies have reported the antifungal activity of silver nanoparticles (AgNPs) against some Candida species. However, ultrastructural analyses on the interaction of AgNPs with these microorganisms have not been reported. In this work we evaluated the effect of AgNPs on C. albicans, and the minimum inhibitory concentration (MIC) was found to have a fungicidal effect. The IC50 was also determined, and the use of AgNPs with fluconazole (FLC), a fungistatic drug, reduced cell proliferation. In order to understand how AgNPs interact with living cells, the ultrastructural distribution of AgNPs in this fungus was determined. Transmission electron microscopy (TEM) analysis revealed a high accumulation of AgNPs outside the cells but also smaller nanoparticles (NPs) localized throughout the cytoplasm. Energy dispersive spectroscopy (EDS) analysis confirmed the presence of intracellular silver. From our results it is assumed that AgNPs used in this study do not penetrate the cell, but instead release silver ions that infiltrate into the cell leading to the formation of NPs through reduction by organic compounds present in the cell wall and cytoplasm."
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.classificationOTRAS ESPECIALIDADES TECNOLÓGICAS
dc.titleUltrastructural analysis of Candida albicans when exposed to silver nanoparticles
dc.typearticle
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0108876
dc.rights.accessAcceso Abierto


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional