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Replicative and integrative plasmids for production of human interferon gamma in Bacillus subtilis

dc.contributor.authorRojas Contreras, Juan Antonio
dc.contributor.authorPedraza Reyes, Mario
dc.contributor.authorOrdoñez Acevedo, Leandro Gabriel
dc.contributor.authorUrtiz Estrada, Norma
dc.contributor.authorBarba de la Rosa, Ana Paulina
dc.contributor.authorDe León Rodríguez, Antonio
dc.contributor.editorElsevier
dc.date.accessioned2018-06-07T20:16:52Z
dc.date.available2018-06-07T20:16:52Z
dc.date.issued2010-11
dc.identifier.citationJuan A. Rojas Contreras, Mario Pedraza-Reyes, Leandro G. Ordoñez, Norma Urtiz Estrada, Ana P. Barba de la Rosa, Antonio De León-Rodríguez, Replicative and integrative plasmids for production of human interferon gamma in Bacillus subtilis, Plasmid, Volume 64, Issue 3, 2010, Pages 170-176.
dc.identifier.urihttp://hdl.handle.net/11627/3873
dc.description.abstract"Integrative and replicative plasmids for the expression driven by the P43 promoter and secretion of recombinant proteins in Bacillus subtilis were constructed. The plasmids named pInt and pRep respectively were tested for the production of recombinant human interferon gamma (rhIFN-γ). A synthetic hIFN-γ gene employing the optimized B. subtilis codon usage was fused with the Bacillus licheniformis α-amylase signal peptide (sp-amyL) encoding sequence. The integrative construct produced 2.5 ± 0.2 mg l−1 and the replicative system produced 20.3 ± 0.8 mg l−1 of total recombinant rhIFN-γ. The results showed that secretion of hIFN-γ was the bottleneck for the overexpression of mature rhIFN-γ by B. subtilis."
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectHeterologous
dc.subjectIntegrative
dc.subjectProbiotic
dc.subjectTherapeutic
dc.subjectRecombinant
dc.subjectReplicative plasmid and secretion
dc.subject.classificationMICROBIOLOGÍA
dc.titleReplicative and integrative plasmids for production of human interferon gamma in Bacillus subtilis
dc.typearticle
dc.identifier.doihttps://doi.org/10.1016/j.plasmid.2010.07.003
dc.rights.accessAcceso Abierto


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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