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Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica

dc.contributor.authorGarcía Echauri, Sergio Ángel
dc.contributor.authorOrdoñez Acevedo, Leandro Gabriel
dc.contributor.authorRojas Contreras, Juan Antonio
dc.contributor.authorBarba de la Rosa, Ana Paulina
dc.contributor.authorDe León Rodríguez, Antonio
dc.date.accessioned2018-06-07T20:16:52Z
dc.date.available2018-06-07T20:16:52Z
dc.date.issued2009-09
dc.identifier.citationSergio A. García Echauri, Manuel Gidekel, Ana Gutiérrez Moraga, Leandro G. Ordóñez, Juan A. Rojas Contreras, Ana P. Barba de la Rosa, Antonio De León Rodríguez, Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica, Process Biochemistry, Volume 44, Issue 9, 2009, Pages 969-974.
dc.identifier.urihttp://hdl.handle.net/11627/3874
dc.description.abstract"Superoxide dismutase (SOD) catalyzes the conversion of the superoxide radical (O2−) into oxygen and hydrogen peroxide. Deschampsia antarctica is a plant that grows in Antarctica and survives to extreme low temperature and high UV radiation, thus it is an ideal model to study novel antioxidants. A cDNA Cu/Zn-SOD gene from D. antarctica was cloned into a pET vector and expressed in Escherichia coli BL21-SI. 112 mg/L of recombinant Cu/Zn-SOD was attained in batch cultures in bioreactor. Using Ni-affinity gel chromatography, the recombinant Cu/Zn-SOD was recovered with a purity of 90% and a specific enzyme activity of 749 at 25 °C. However, zymogram test showed that the enzyme has more activity at 4 °C. This D. antarctica SOD could be used to reduce the oxidation of refrigerated and frozen foods."
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAntioxidant
dc.subjectPlant
dc.subjectExtremophil
dc.subjectPsychrophilic enzyme
dc.subjectPhoto-oxidation
dc.subjectOxidative stress
dc.subject.classificationCIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA
dc.titleHeterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica
dc.typearticle
dc.identifier.doihttps://doi.org/10.1016/j.procbio.2009.04.021
dc.rights.accessAcceso Abierto


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional