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Expansion of human hematopoietic cells from umbilical cord blood using roller bottles in CO2 and CO2-free atmosphere

dc.contributor.authorAndrade Zaldívar, Hera
dc.contributor.authorKalixto Sánchez, Marco Antonio
dc.contributor.authorBarba de la Rosa, Ana Paulina
dc.contributor.authorDe León Rodríguez, Antonio
dc.contributor.editorMary Ann Liebert, Inc
dc.date.accessioned2018-06-07T20:16:55Z
dc.date.available2018-06-07T20:16:55Z
dc.date.issued2011-04
dc.identifier.citationHera Andrade-Zaldívar, Marco A. Kalixto-Sánchez, Ana P. Barba de la Rosa, and Antonio De León-Rodríguez.Stem Cells and Development.Apr 2011.ahead of printhttp://doi.org/10.1089/scd.2010.0236
dc.identifier.urihttp://hdl.handle.net/11627/3879
dc.description.abstract"In this work, we evaluated the expansion of human hematopoietic stem cells from umbilical cord blood in roller bottles. The Iscove's modified Dulbecco's medium, the Stem Pro 34-SFM medium, and the L-15 Leibovitz's medium for cultures in CO2-free atmosphere were assessed. At day 5 of culture, total colony forming unit expansions of 14.44 ± 3.74, 11.20 ± 6.37, and 17.25 ± 3.65-folds were attained, respectively. The expansion reached using L-15 medium in roller bottles was around 10 times higher than that achieved in the static control cultures. To our knowledge, this is the first report of cultures in CO2-free atmosphere to expand cord blood human hematopoietic stem cells and it opens a new branch of possibilities for culturing and clinical applications."
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.classificationBIOLOGÍA CELULAR
dc.titleExpansion of human hematopoietic cells from umbilical cord blood using roller bottles in CO2 and CO2-free atmosphere
dc.typearticle
dc.identifier.doihttps://doi.org/10.1089/scd.2010.0236
dc.rights.accessAcceso Abierto


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional