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Dual function of EDTA with silver nanoparticles for root canal treatment-A novel modification

dc.contributor.authorMartínez Andrade, Juan Manuel
dc.contributor.authorÁvalos Borja, Miguel
dc.contributor.authorVilchis Nestor, Alfredo Rafael
dc.contributor.authorSánchez Vargas, Luis Octavio
dc.contributor.authorCastro Longoria, Ernestina
dc.contributor.editorPublic Library of Science
dc.date.accessioned2018-12-13T21:09:35Z
dc.date.available2018-12-13T21:09:35Z
dc.date.issued2018
dc.identifier.citationMartinez-Andrade JM, Avalos-Borja M, Vilchis-Nestor AR, Sanchez-Vargas LO, Castro-Longoria E (2018) Dual function of EDTA with silver nanoparticles for root canal treatment-A novel modification. PLoS ONE 13(1): e0190866. https://doi.org/10.1371/journal.pone.0190866
dc.identifier.urihttp://hdl.handle.net/11627/4833
dc.description.abstract"The chelating and antimicrobial capacity of a novel modification of 17% EDTA with silver nanoparticles (AgNPs) (EDTA-AgNPs) was evaluated in-vitro for root canal treatment (RCT). The EDTA-AgNPs solution was characterized by UV-Vis spectroscopy, ζ-potential and high-resolution transmission electron microscopy (HRTEM). Antimicrobial capacity was evaluated against Candida albicans and Staphylococcus aureus in planktonic and biofilm cells by broth macrodilution (24 h) and XTT assays, (1, 10 and 30 min) respectively. The chelating capacity of EDTA-AgNPs was assessed indirectly (smear layer removal) and directly (demineralizing effect) in bovine dentin at two silver concentrations, 16 and 512 μg/ml at 1 and 10 minutes of exposure time. Smear layer removal was evaluated by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The demineralizing effect was determined by atomic absorption spectroscopy (AAS), microhardness test (MH) and X-ray diffractometer (XRD). Synthesized AgNPs were quasi-spherical in shape with an average size of 13.09 ± 8.05 nm. 17% EDTA-AgNPs was effective to inhibit C. albicans and S. aureus in planktonic and biofilm cultures. The smear layer removal and demineralizing effect were similar between 17% EDTA-AgNPs and 17% EDTA treatments. The 17% EDTA-AgNPs solution proved to be an effective antimicrobial agent, and has a similar chelating capacity to 17% EDTA alone. These in-vitrostudies strongly suggest that EDTA-AgNPs could be used for effective smear layer removal, having an antimicrobial effect at the same time during RCT."
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.classificationCIENCIAS TECNOLÓGICAS
dc.titleDual function of EDTA with silver nanoparticles for root canal treatment-A novel modification
dc.typearticle
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0190866
dc.rights.accessAcceso Abierto


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional