Show simple item record

Title

The corn smut-made cholera oral vaccine is thermostable and induces long-lasting immunity in mouse

dc.contributor.authorMonreal Escalante, Elizabeth
dc.contributor.authorNavarro Tovar, Gabriela
dc.contributor.authorLeón Gallo, Amalia
dc.contributor.authorJuárez Montiel, Margarita
dc.contributor.authorBecerra Flora, Alicia
dc.contributor.authorJiménez Bremont, Juan Francisco
dc.contributor.authorRosales Mendoza, Sergio
dc.date.accessioned2019-08-09T22:22:44Z
dc.date.available2019-08-09T22:22:44Z
dc.date.issued2016
dc.identifier.citationElizabeth Monreal-Escalante, Gabriela Navarro-Tovar, Amalia León-Gallo, Margarita Juárez-Montiel, Alicia Becerra-Flora, Juan Francisco Jiménez-Bremont, Sergio Rosales-Mendoza, The corn smut-made cholera oral vaccine is thermostable and induces long-lasting immunity in mouse, Journal of Biotechnology, Volume 234, 2016, Pages 1-6.
dc.identifier.urihttp://hdl.handle.net/11627/5035
dc.description.abstract"The use of corn smut for the production of recombinant vaccines has been recently implemented by our group. In this study, the stability and immunogenic properties of the corn smut-based cholera vaccine, based on the cholera toxin B subunit (CTB), were determined in mouse. The immunogenic potential of distinct corn smut CTB doses ranging from 1 to 30 ?g were assessed, with maximum humoral responses at both the systemic (IgG) and intestinal (IgA) levels at a dose of 15 ?g. The humoral response last for up to 70 days after the third boost. Mice were fully protected against a challenge with cholera toxin after receiving three 15 ?g-doses. Remarkably, the corn smut-made vaccine retained its immunogenic activity after storage at room temperature for a period of 1 year and no reduction on CTB was observed following exposure at 50 °C for 2 h. These data support the use of the corn smut-made CTB vaccine as a highly stable and effective immunogen and justify its evaluation in target animal models, such as piglet and sheep, as well as clinical evaluations in humans."
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCholera
dc.subjectLow-cost vaccine
dc.subjectOral immunization
dc.subjectCold chain
dc.subjectNeutralizing antibodies
dc.subjectCholera toxin
dc.subject.classificationMICROBIOLOGÍA
dc.titleThe corn smut-made cholera oral vaccine is thermostable and induces long-lasting immunity in mouse
dc.typearticle
dc.identifier.doihttps://doi.org/10.1016/j.jbiotec.2016.04.047
dc.rights.accessAcceso Abierto


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional