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Removing lateral chromatic aberration in bright field optical microscopy

dc.contributor.authorGuzmán Altamirano, Miguel Angel
dc.contributor.authorGutiérrez Medina, Braulio
dc.date.accessioned2019-08-09T22:22:53Z
dc.date.available2019-08-09T22:22:53Z
dc.date.issued2015
dc.identifier.citationMiguel Guzmán-Altamirano and Braulio Gutiérrez-Medina, "Removing lateral chromatic aberration in bright field optical microscopy," Opt. Express 23, 14380-14390 (2015)
dc.identifier.urihttp://hdl.handle.net/11627/5057
dc.description.abstract"We present an efficient alternative to remove lateral chromatic aberration (LCA) in bright field light microscopy images. Our procedure is based on error calibration using time-sequential acquisition at different wavelengths, and error correction through digital image warping. Measurement of the displacements of fiducial marks in the red and green images relative to blue provide calibration factors that are subsequently used in test images to realign color channels digitally. We demonstrate quantitative improvement in the position and boundaries of objects in target slides and in the color content and morphology of specimens in stained biological samples. Our results show a reduction of LCA content below the 0.1% level. "
dc.publisherOptical Society
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectFluorescence microscopu
dc.subject.classificationÓPTICA
dc.titleRemoving lateral chromatic aberration in bright field optical microscopy
dc.typearticle
dc.identifier.doihttps://doi.org/10.1364/OE.23.014380
dc.rights.accessAcceso Abierto


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional