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Diversification of transcriptional regulation determines subfunctionalization of paralogous branched chain aminotransferases in the yeast Saccharomyces cerevisiae

dc.contributor.authorGonzález Flores, James
dc.contributor.authorLópez, Geovani
dc.contributor.authorArgueta, Stefany
dc.contributor.authorEscalera-Fanjul, Ximena
dc.contributor.authorHafidi, Mohammed El
dc.contributor.authorCampero Basaldúa, José Carlos
dc.contributor.authorStrauss, Joseph
dc.contributor.authorRiego Ruíz, Lina Raquel
dc.contributor.authorGonzález Manjarrez, María Alicia
dc.date.accessioned2019-08-23T22:04:56Z
dc.date.available2019-08-23T22:04:56Z
dc.date.issued2017
dc.identifier.citationGonzález, J., López, G., Argueta, S., Escalera-Fanjul, X., El Hafidi, M., Campero-Basaldua, C., … González, A. (2017). Diversification of Transcriptional Regulation Determines Subfunctionalization of Paralogous Branched Chain Aminotransferases in the Yeast Saccharomyces cerevisiae. Genetics, 207(3), 975–991. doi:10.1534/genetics.117.300290
dc.identifier.urihttp://hdl.handle.net/11627/5113
dc.description.abstract"Saccharomyces cerevisiae harbors BAT1 and BAT2 paralogous genes that encode branched chain aminotransferases and have opposed expression profiles and physiological roles . Accordingly, in primary nitrogen sources such as glutamine, BAT1 expression is induced, supporting Bat1-dependent valine–isoleucine–leucine (VIL) biosynthesis, while BAT2 expression is repressed. Conversely, in the presence of VIL as the sole nitrogen source, BAT1 expression is hindered while that of BAT2 is activated, resulting in Bat2-dependent VIL catabolism. The presented results confirm that BAT1 expression is determined by transcriptional activation through the action of the Leu3–?-isopropylmalate (?-IPM) active isoform, and uncovers the existence of a novel ?-IPM biosynthetic pathway operating in a put3? mutant grown on VIL, through Bat2-Leu2-Leu1 consecutive action. The classic ?-IPM biosynthetic route operates in glutamine through the action of the leucine-sensitive ?-IPM synthases. The presented results also show that BAT2 repression in glutamine can be alleviated in a ure2? mutant or through Gcn4-dependent transcriptional activation. Thus, when S. cerevisiae is grown on glutamine, VIL biosynthesis is predominant and is preferentially achieved through BAT1; while on VIL as the sole nitrogen source, catabolism prevails and is mainly afforded by BAT2."
dc.publisherGenetics Society of America
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectFunctional diversification
dc.subjectExpression regulation
dc.subjectAminotransferases
dc.subjectAmino acid metabolism
dc.subjectParalogous genes
dc.subject.classificationGENÉTICA
dc.titleDiversification of transcriptional regulation determines subfunctionalization of paralogous branched chain aminotransferases in the yeast Saccharomyces cerevisiae
dc.typearticle
dc.identifier.doihttps://doi.org/10.1534/genetics.117.300290
dc.rights.accessAcceso Abierto


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional