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Generation of Sporothrix schenckii mutants expressing the green fluorescent protein suitable for the study of host-fungus interactions

dc.contributor.authorLozoya Pérez, Nancy Edith
dc.contributor.authorCasas Flores, J. Sergio
dc.contributor.authorMartínez Álvarez, José Ascención
dc.contributor.authorLópez-Ramírez, Luz Adriana
dc.contributor.authorLópes Bezerra, Leila Maria
dc.contributor.authorFranco, Bernardo
dc.contributor.authorMora Montes, Héctor Manuel
dc.date.accessioned2019-08-30T22:43:25Z
dc.date.available2019-08-30T22:43:25Z
dc.date.issued2018
dc.identifier.citationNancy E. Lozoya-Pérez, Sergio Casas-Flores, José A. Martínez-Álvarez, Luz A. López-Ramírez, Leila M. Lopes-Bezerra, Bernardo Franco, Héctor M. Mora-Montes, Generation of Sporothrix schenckii mutants expressing the green fluorescent protein suitable for the study of host-fungus interactions, Fungal Biology, Volume 122, Issue 10, 2018, Pages 1023-1030.
dc.identifier.urihttp://hdl.handle.net/11627/5168
dc.description.abstract"Sporotrichosis is an infection caused by members of the Sporothrix genus, and among them, Sporothrix schenckii is one of the etiological agents. Both, the disease and the causative agent have gained interest in the recent years, because of the report of epidemic outbreaks, and the description of the disease transmission from animals to human beings. Despite the relevance of S. schenckii in the clinical field, there are basic aspects of its biology poorly explored. So far, Agrobacterium tumefaciens-mediated transformation has been reported as an alternative for genetic manipulation of this fungal pathogen. Here, we report the optimization of the transformation method and used this to generate insertional mutants that express the green fluorescent protein in S. schenckii. We obtained five mutant strains that showed mitotic stability and expression of the reporter gene. The strains displayed normal cell wall composition, and a similar ability to interact ex vivo with human monocytes and monocyte-derived macrophages. Moreover, the virulence in larvae of Galleria mellonella was similar to that obtained with the wild-type control strains. These data indicate that these fluorescent mutants with normal ability to interact with the host could be used in bioimaging to track the host-Sporothrix interaction in vivo."
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAgrobacterium tumefaciens
dc.subjectBioimaging
dc.subjectCell wall
dc.subjectImmune sensing
dc.subjectTransformation
dc.subjectVirulence
dc.subject.classificationMICOLOGÍA (SETAS)
dc.titleGeneration of Sporothrix schenckii mutants expressing the green fluorescent protein suitable for the study of host-fungus interactions
dc.typearticle
dc.identifier.doihttps://doi.org/10.1016/j.funbio.2018.07.004
dc.rights.accessAcceso Abierto


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional