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Molecular characterization of the silencing complex SIR in Candida glabrata hyperadherent clinical isolates

dc.contributor.authorLeiva Peláez, Osney
dc.contributor.authorGutiérrez Escobedo, Ma Guadalupe
dc.contributor.authorLópez-Fuentes, Eunice
dc.contributor.authorCruz Mora, José
dc.contributor.authorDe Las Peñas Nava, Alejandro
dc.contributor.authorCastaño Navarro, Irene Beatriz
dc.date.accessioned2019-08-30T22:43:26Z
dc.date.available2019-08-30T22:43:26Z
dc.date.issued2018
dc.identifier.citationOsney Leiva-Peláez, Guadalupe Gutiérrez-Escobedo, Eunice López-Fuentes, José Cruz-Mora, Alejandro De Las Peñas, Irene Castaño, Molecular characterization of the silencing complex SIR in Candida glabrata hyperadherent clinical isolates, Fungal Genetics and Biology, Volume 118, 2018, Pages 21-31.
dc.identifier.urihttp://hdl.handle.net/11627/5169
dc.description.abstract"An important virulence factor for the fungal pathogen Candida glabrata is the ability to adhere to the host cells, which is mediated by the expression of adhesins. Epa1 is responsible for ?95% of the in vitro adherence to epithelial cells and is the founding member of the Epa family of adhesins. The majority of EPA genes are localized close to different telomeres, which causes transcriptional repression due to subtelomeric silencing. In C. glabrata there are three Sir proteins (Sir2, Sir3 and Sir4) that are essential for subtelomeric silencing. Among a collection of 79 clinical isolates, some display a hyperadherent phenotype to epithelial cells compared to our standard laboratory strain, BG14. These isolates also express several subtelomeric EPA genes simultaneously. We cloned the SIR2, SIR3 and SIR4 genes from the hyperadherent isolates and from the BG14 and the sequenced strain CBS138 in a replicative vector to complement null mutants in each of these genes in the BG14 background. All the SIR2 and SIR4 alleles tested from selected hyper-adherent isolates were functional and efficient to silence a URA3 reporter gene inserted in a subtelomeric region. The SIR3 alleles from these isolates were also functional, except the allele from isolate MC2 (sir3-MC2), which was not functional to silence the reporter and did not complement the hyperadherent phenotype of the BG14 sir3?. Consistently, sir3-MC2 allele is recessive to the SIR3 allele from BG14. Sir3 and Sir4 alleles from the hyperadherent isolates contain several polymorphisms and two of them are present in all the hyperadherent isolates analyzed. Instead, the Sir3 and Sir4 alleles from the BG14 and another non-adherent isolate do not display these polymorphisms and are identical to each other. The particular combination of polymorphisms in sir3-MC2 and in SIR4-MC2 could explain in part the hyperadherent phenotype displayed by this isolate."
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCandida glabrata
dc.subjectHyperadherence
dc.subjectClinical isolates
dc.subjectSIR complex
dc.subjectPolymorphisms
dc.subjectSIR3
dc.subjectSIR4
dc.subject.classificationMICOLOGÍA (LEVADURAS)
dc.titleMolecular characterization of the silencing complex SIR in Candida glabrata hyperadherent clinical isolates
dc.typearticle
dc.identifier.doihttps://doi.org/10.1016/j.fgb.2018.05.005
dc.rights.accessAcceso Abierto


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional