Show simple item record

Title

PEST sequences from a cactus dehydrin regulate its proteolytic degradation

dc.contributor.authorSalazar Retana, Adriana Leticiaes_MX
dc.contributor.authorMaruri López, Israeles_MX
dc.contributor.authorHernández Sánchez, Itzell Eurídicees_MX
dc.contributor.authorBecerra Flora, Aliciaes_MX
dc.contributor.authorGuerrero González, Maria de la Luzes_MX
dc.contributor.authorJiménez Bremont, Juan Franciscoes_MX
dc.date.accessioned2020-03-04T00:53:32Z
dc.date.available2020-03-04T00:53:32Z
dc.date.issued2019
dc.identifier.citationSalazar-Retana AL, Maruri-López I, Hernández-Sánchez IE, Becerra-Flora A, Guerrero-González MdlL, Jiménez-Bremont JF. 2019. PEST sequences from a cactus dehydrin regulate its proteolytic degradation. PeerJ 7:e6810 https://doi.org/10.7717/peerj.6810es_MX
dc.identifier.urihttp://hdl.handle.net/11627/5297
dc.description.abstract"Dehydrins (DHNs) are intrinsically disordered proteins expressed under cellular dehydration-related stresses. In this study, we identified potential proteolytic PEST sequences located at the central and C-terminal regions from the Opuntia streptacantha OpsDHN1 protein. In order to evaluate these PEST sequences as proteolytic tags, we generated a translational fusion with the GUS reporter protein and OpsDHN1 coding sequence. We found a GUS degradation effect in tobacco agro-infiltrated leaves and Arabidopsis transgenic lines that expressed the fusion GUS::OpsDHN1 full-length. Also, two additional translational fusions between OpsDHN1 protein fragments that include the central (GUS::PEST-1) or the C-terminal (GUS::PEST-2) PEST sequences were able to decrease the GUS activity, with PEST-2 showing the greatest reduction in GUS activity. GUS signal was abated when the OpsDHN1 fragment that includes both PEST sequences (GUS::PEST-1-2) were fused to GUS. Treatment with the MG132 proteasome inhibitor attenuated the PEST-mediated GUS degradation. Point mutations of phosphorylatable residues in PEST sequences reestablished GUS signal, hence these sequences are important during protein degradation. Finally, in silico analysis identified potential PEST sequences in other plant DHNs. This is the first study reporting presence of PEST motifs in dehydrins."
dc.description.abstract"Dehydrins (DHNs) are intrinsically disordered proteins expressed under cellular dehydration-related stresses. In this study, we identified potential proteolytic PEST sequences located at the central and C-terminal regions from the Opuntia streptacantha OpsDHN1 protein. In order to evaluate these PEST sequences as proteolytic tags, we generated a translational fusion with the GUS reporter protein and OpsDHN1 coding sequence. We found a GUS degradation effect in tobacco agro-infiltrated leaves and Arabidopsis transgenic lines that expressed the fusion GUSes_MX
dc.publisherPeerJ, Inc
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectDehydrines_MX
dc.subjectIntrinsically disordered proteinses_MX
dc.subjectPest sequenceses_MX
dc.subjectProtein degradationes_MX
dc.subject.classificationBIOLOGÍA MOLECULARes_MX
dc.titlePEST sequences from a cactus dehydrin regulate its proteolytic degradationes_MX
dc.typearticlees_MX
dc.identifier.doihttps://doi.org/10.7717/peerj.6810
dc.rights.accessAcceso Abierto


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional