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Modulation of Caecal Microbiome in Obese Mice Associated with Administration of Amaranth or Soybean Protein Isolates

dc.contributor.authorOlguín Calderón, Diana
dc.contributor.authorGonzález Escobar, Jorge Luis
dc.contributor.authorRíos Villa, Rosalva
dc.contributor.authorDibildox Alvarado, Elena
dc.contributor.authorDe León Rodríguez, Antonio
dc.contributor.authorBarba de la Rosa, Ana Paulina
dc.date.accessioned2020-03-04T00:53:33Z
dc.date.available2020-03-04T00:53:33Z
dc.date.issued2019
dc.identifier.citationOlguín-Calderón, D., González-Escobar, J. L., Ríos-Villa, R., Dibildox-Alvarado, E., De León-Rodríguez, A., Barba de la Rosa, A. P. (2019). Modulation of Caecal Microbiome in Obese Mice Associated with Administration of Amaranth or Soybean Protein Isolates. Polish Journal of Food and Nutrition Sciences, 69(1), 35-44. https://doi.org/10.31883/pjfns-2019-0002
dc.identifier.urihttp://hdl.handle.net/11627/5299
dc.description.abstract"Obesity is defined as abnormal or excessive body fat accumulation that may have negative effects on health. Healthy diet induces a balance of gut microbiota, helping in turn to combat this metabolic disorder. Amaranth is well known because of its beneficial properties on health. but its effects on microbiota profile are still unknown. The aim of this study was to analyse the changes of gut microbiota in diet-induced obese mice due to amaranth protein consumption and to compare them with the changes due to soybean protein intake. Male C57BL/6 mice were fed for 8 weeks with regular (RD) or high-fat (HF) diet, without or with complementation with amaranth or soybean protein isolates. Morphological changes in caecum ultrathin sections were measured after hematoxylin/eosin staining. Microbiota was isolated from the caecum and 16S rRNA gene was sequenced. Caecal Short Chain Fatty Acids (SCEs) were quantified by gas chromatography. The consumption of soybean protein induced the ectopic deposition of fat in the whole intestine while amaranth proteins increased caecal crypt depth and calceiform cells number sustaining its beneficial effect on health. The count of Ruminococcacea family bacteria was increased in mice fed with HF diet, but amaranth proteins intake reduced its abundance. In turn, Lachnospiraceae bacteria abundance decreased in mice fed the Control-HF and amaranth HF diets. but increased in mice fed the soybean diets. In mice fed the soybean diets. amaranth induced the abundance of Prevotellaceae, an acetate-producing bacteria. Study results indicate that the modulation of caecal microbiota could be one of the mechanisms by which amaranth exerts its beneficial effects on health."
dc.publisherInstitute of Animal Reproduction and Food Research of the Polish Academy of Sciences
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAmaranth
dc.subjectCaecal microbiome
dc.subjectObesity
dc.subjectProtein intake
dc.subjectShort-chain fatty acids
dc.subject.classificationNUTRICIÓN
dc.titleModulation of Caecal Microbiome in Obese Mice Associated with Administration of Amaranth or Soybean Protein Isolates
dc.typearticle
dc.identifier.doihttps://doi.org/10.31883/pjfns-2019-0002
dc.rights.accessAcceso Abierto


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional