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Secretion of an argonaute protein by a parasitic nematode and the evolution of its siRNA guides

dc.contributor.authorWang-Ngai Chow, Franklin
dc.contributor.authorKoutsovoulos, Georgios
dc.contributor.authorOvando Vázquez, Cesaré Moises
dc.contributor.authorNeophytou, Kyriaki
dc.contributor.authorBermúdez Barrientos, José Roberto
dc.contributor.authorLaetsch, Dominik R.
dc.contributor.authorRobertson, Elaine
dc.contributor.authorKumar, Sujai
dc.contributor.authorClaycomb, Julie M.
dc.contributor.authorBlaxter, Mark
dc.contributor.authorAbreu Goodger, Cei
dc.contributor.authorBuck, Amy H.
dc.date.accessionedM
dc.date.available2020-03-10T20:43:57Z
dc.date.issued2019
dc.identifier.citationFranklin Wang-Ngai Chow, Georgios Koutsovoulos, Cesaré Ovando Vázquez, Kyriaki Neophytou, Jose R Bermúdez-Barrientos, Dominik R Laetsch, Elaine Robertson, Sujai Kumar, Julie M Claycomb, Mark Blaxter, Cei Abreu-Goodger, Amy H Buck, Secretion of an Argonaute protein by a parasitic nematode and the evolution of its siRNA guides, Nucleic Acids Research, Volume 47, Issue 7, 23 April 2019, Pages 3594-3606, https://doi.org/10.1093/nar/gkz142
dc.identifier.urihttp://hdl.handle.net/11627/5306
dc.description.abstract"Extracellular RNA has been proposed to mediate communication between cells and organisms however relatively little is understood regarding how specific sequences are selected for export. Here, we describe a specific Argonaute protein (exWAGO) that is secreted in extracellular vesicles (EVs) released by the gastrointestinal nematode Heligmosomoides bakeri, at multiple copies per EV. Phylogenetic and gene expression analyses demonstrate exWAGO orthologues are highly conserved and abundantly expressed in related parasites but highly diverged in free-living genus Caenorhabditis. We show that the most abundant small RNAs released from the nematode parasite are not microRNAs as previously thought, but rather secondary small interfering RNAs (siRNAs) that are produced by RNA-dependent RNA Polymerases. The siRNAs that are released in EVs have distinct evolutionary properties compared to those resident in free-living or parasitic nematodes. Immunoprecipitation of exWAGO demonstrates that it specifically associates with siRNAs from transposons and newly evolved repetitive elements that are packaged in EVs and released into the host environment. Together this work demonstrates molecular and evolutionary selectivity in the small RNA sequences that are released in EVs into the host environment and identifies a novel Argonaute protein as the mediator of this."
dc.publisherOxford University Press
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectWeb server
dc.subjectRNA
dc.subjectAnnotation
dc.subject.classificationBIOQUÍMICA
dc.titleSecretion of an argonaute protein by a parasitic nematode and the evolution of its siRNA guides
dc.typearticle
dc.identifier.doihttps://doi.org/10.1093/nar/gkz142
dc.rights.accessAcceso Abierto


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional