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Decrease of Arabidopsis PAO activity entails increased RBOH activity, ROS content and altered responses to Pseudomonas

dc.contributor.authorJasso Robles, Francisco Ignacio
dc.contributor.authorGonzález, María Elisa
dc.contributor.authorPieckenstain, Fernando Luis
dc.contributor.authorRamírez García, José Miguel
dc.contributor.authorGuerrero González, Maria de la Luz
dc.contributor.authorJiménez Bremont, Juan Francisco
dc.contributor.authorRodríguez y Domínguez Kessler, Margarita
dc.date.accessioned2021-06-10T20:08:22Z
dc.date.available2021-06-10T20:08:22Z
dc.date.issued2020
dc.identifier.citationFrancisco Ignacio Jasso-Robles, María Elisa Gonzalez, Fernando Luis Pieckenstain, José Miguel Ramírez-García, María de la Luz Guerrero-González, Juan Francisco Jiménez-Bremont, Margarita Rodríguez-Kessler, Decrease of Arabidopsis PAO activity entails increased RBOH activity, ROS content and altered responses to Pseudomonas, Plant Science, Volume 292, 2020, 110372, https://doi.org/10.1016/j.plantsci.2019.110372.
dc.identifier.urihttp://hdl.handle.net/11627/5639
dc.description.abstract"Polyamines (PAs) are small aliphatic amines with important regulatory activities in plants. Biotic stress results in changes in PA levels due to de novo synthesis and PA oxidation. In Arabidopsis thaliana five FAD-dependent polyamine oxidase enzymes (AtPAO1-5) participate in PA back-conversion and degradation. PAO activity generates H2O2, an important molecule involved in cell signaling, elongation, programmed cell death, and defense responses. In this work we analyzed the role of AtPAO genes in the Arabidopsis thaliana-Pseudomonas syringae pathosystem. AtPAO1 and AtPAO2 genes were transcriptionally up-regulated in infected plants. Atpao1-1 and Atpao2-1 single mutant lines displayed altered responses to Pseudomonas, and an increased susceptibility was found in the double mutant Atpao1-1 x Atpao2-1. These polyamine oxidases mutant lines showed disturbed contents of ROS (H2O2 and O-2(center dot-)) and altered activities of RBOH, CAT and SOD enzymes both in infected and control plants. In addition, changes in the expression levels of AtRBOHD, AtRBOHF, AtPRX33, and AtPRX34 genes were also noticed. Our data indicate an important role for polyamine oxidases in plant defense and ROS homeostasis."
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectArabidopsis thaliana-Pseudomonas syringae pathosystem
dc.subjectHydrogen peroxide
dc.subjectPolyamine oxidases
dc.subjectSuperoxide anion radical
dc.subjectRBOH enzymes
dc.subjectSpermine
dc.subject.classificationBIOQUÍMICA
dc.titleDecrease of Arabidopsis PAO activity entails increased RBOH activity, ROS content and altered responses to Pseudomonas
dc.typearticle
dc.identifier.doihttps://doi.org/10.1016/j.plantsci.2019.110372
dc.rights.accessAcceso Abierto


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