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The Banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 Genes Encode Functional Transcription Factors and Display Differential Expression in Response to Defense Phytohormones

dc.contributor.authorGarcía Laynes, Sergio
dc.contributor.authorHerrera-Valencia, Virginia Aurora
dc.contributor.authorTamayo Torres, Lilia Guadalupe
dc.contributor.authorLimones Briones, Verónica
dc.contributor.authorBarredo Pool, Felipe Alonso
dc.contributor.authorBaas Espinola, Fray Martin
dc.contributor.authorAlpuche Solís, Ángel Gabriel
dc.contributor.authorPuch Hau, Carlos
dc.contributor.authorPeraza Echeverria, Santy
dc.date.accessioned2023-06-14T16:12:23Z
dc.date.available2023-06-14T16:12:23Z
dc.date.issued2022
dc.identifier.citationGarcía-Laynes, S.; Herrera-Valencia, V.A.; Tamayo-Torres, L.G.; Limones-Briones, V.; Barredo-Pool, F.A.; Baas-Espinola, F.M.; Alpuche-Solís, A.G.; Puch-Hau, C.; Peraza-Echeverria, S. The Banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 Genes Encode Functional Transcription Factors and Display Differential Expression in Response to Defense Phytohormones. Genes 2022, 13, 1891. https://doi.org/10.3390/genes13101891
dc.identifier.urihttp://hdl.handle.net/11627/6333
dc.description.abstract"WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and Arabidopsis WRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop."
dc.publisherMDPI
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBanana
dc.subjectTranscription factor
dc.subjectWRKY
dc.subjectDefense phytohormones
dc.subjectSalicylic acid
dc.subjectMethyl jasmonate
dc.subjectSAR
dc.subjectISR
dc.subjectBroad-spectrum resistance
dc.subject.classificationGENÉTICA
dc.titleThe Banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 Genes Encode Functional Transcription Factors and Display Differential Expression in Response to Defense Phytohormones
dc.typearticle
dc.identifier.doihttps://doi.org/10.3390/genes13101891
dc.rights.accessAcceso Abierto


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