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MiDAS 4: A global catalogue of full-length 16S rRNA gene sequences and taxonomy for studies of bacterial communities in wastewater treatment plants

dc.contributor.authorDueholm, Morten Kam Dahl
dc.contributor.authorNierychlo, Marta
dc.contributor.authorAndersen, Kasper Skytte
dc.contributor.authorRudkjøbing, Vibeke
dc.contributor.authorKnutsson, Simon
dc.contributor.authorArriaga García, Sonia Lorena
dc.contributor.authorBakke, Rune
dc.contributor.authorBoon, Nico
dc.contributor.authorBux, Faizal
dc.contributor.authorChristensson, Magnus
dc.contributor.authorChua, Adeline Seak May
dc.contributor.authorCurtis, Thomas P.
dc.contributor.authorCytryn, Eddie
dc.contributor.authorErijman, Leonardo
dc.contributor.authorEtchebehere, Claudia
dc.contributor.authorFatta-Kassinos, Despo
dc.contributor.authorFrigon, Dominic
dc.contributor.authorGarcia Chaves, Maria Carolina
dc.contributor.authorGu, April Z.
dc.contributor.authorHorn, Harald
dc.contributor.authorJenkins, David
dc.contributor.authorKreuzinger, Norbert
dc.contributor.authorKumari, Sheena
dc.contributor.authorLanham, Ana
dc.contributor.authorLaw, Yingyu
dc.contributor.authorLeiknes, TorOve
dc.contributor.authorMorgenroth, Eberhard
dc.contributor.authorMuszy?ski, Adam
dc.contributor.authorPetrovski, Steve
dc.contributor.authorPijuan, Maite
dc.contributor.authorPillai, Suraj Babu
dc.contributor.authorReis, Maria A. M.
dc.contributor.authorRong, Qi
dc.contributor.authorRossetti, Simona
dc.contributor.authorSeviour, Robert
dc.contributor.authorTooker, Nick
dc.contributor.authorVainio, Pirjo
dc.contributor.authorvan Loosdrecht, Mark
dc.contributor.authorVikraman R.
dc.contributor.authorWanner, Ji?í
dc.contributor.authorWeissbrodt, David
dc.contributor.authorWen, Xianghua
dc.contributor.authorZhang, Tong
dc.contributor.authorNielsen, Per H.
dc.contributor.authorAlbertsen, Mads
dc.contributor.authorNielsen, Per Halkjær
dc.date.accessioned2023-06-14T16:12:27Z
dc.date.available2023-06-14T16:12:27Z
dc.date.issued2022
dc.identifier.citationDueholm, M.K.D., Nierychlo, M., Andersen, K.S. et al. MiDAS 4: A global catalogue of full-length 16S rRNA gene sequences and taxonomy for studies of bacterial communities in wastewater treatment plants. Nat Commun 13, 1908 (2022). https://doi.org/10.1038/s41467-022-29438-7
dc.identifier.urihttp://hdl.handle.net/11627/6347
dc.description.abstract"Microbial communities are responsible for biological wastewater treatment, but our knowledge of their diversity and function is still poor. Here, we sequence more than 5 million high-quality, full-length 16S rRNA gene sequences from 740 wastewater treatment plants (WWTPs) across the world and use the sequences to construct the 'MiDAS 4' database. MiDAS 4 is an amplicon sequence variant resolved, full-length 16S rRNA gene reference database with a comprehensive taxonomy from domain to species level for all sequences. We use an independent dataset (269 WWTPs) to show that MiDAS 4, compared to commonly used universal reference databases, provides a better coverage for WWTP bacteria and an improved rate of genus and species level classification. Taking advantage of MiDAS 4, we carry out an amplicon-based, global-scale microbial community profiling of activated sludge plants using two common sets of primers targeting regions of the 16S rRNA gene, revealing how environmental conditions and biogeography shape the activated sludge microbiota. We also identify core and conditionally rare or abundant taxa, encompassing 966 genera and 1530 species that represent approximately 80% and 50% of the accumulated read abundance, respectively. Finally, we show that for well-studied functional guilds, such as nitrifiers or polyphosphate-accumulating organisms, the same genera are prevalent worldwide, with only a few abundant species in each genus. Microbial communities are responsible for biological wastewater treatment. Here, Dueholm et al. generate more than 5 million high-quality, full-length 16S rRNA gene sequences from wastewater treatment plants across the world to construct a database with a comprehensive taxonomy, providing insights into diversity and function of these microbial communities."
dc.publisherSpringer Nature
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBiological phosphorus removal
dc.subjectAmmonia-oxidizing archaea
dc.subjectComplete nitrification
dc.subjectDiversity
dc.subject.classificationCIENCIAS TECNOLÓGICAS
dc.titleMiDAS 4: A global catalogue of full-length 16S rRNA gene sequences and taxonomy for studies of bacterial communities in wastewater treatment plants
dc.typearticle
dc.identifier.doihttps://doi.org/10.1038/s41467-022-29438-7
dc.rights.accessAcceso Abierto


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