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Standardized protocol for determination of biohydrogen potential

dc.contributor.authorCarrillo Reyes, Julián
dc.contributor.authorBuitron, German
dc.contributor.authorMoreno Andrade, Ivan
dc.contributor.authorTapia Rodríguez, Aida Cecilia
dc.contributor.authorPalomo Briones, Rodolfo
dc.contributor.authorRazo Flores, Elías
dc.contributor.authorAguilar Juárez, Oscar
dc.contributor.authorArreola Vargas, Jorge
dc.contributor.authorBernet, Nicolas
dc.contributor.authorMaluf Braga, Adriana Ferreira
dc.contributor.authorBraga, Lucía
dc.contributor.authorCastelló, Elena
dc.contributor.authorChatellard, Lucile
dc.contributor.authorEtchebehere, Claudia
dc.contributor.authorFuentes, Laura
dc.contributor.authorLeón Becerril, Elizabeth
dc.contributor.authorMéndez Acosta, Hugo Oscar
dc.contributor.authorRuíz Filippi, Gonzalo
dc.contributor.authorTapia Venegas, Estela
dc.contributor.authorTrably, Eric
dc.contributor.authorWenzel, Jorge
dc.contributor.authorZaiat, Marcelo
dc.date.accessioned2021-04-26T19:14:06Z
dc.date.available2021-04-26T19:14:06Z
dc.date.issued2020
dc.identifier.citationJulián Carrillo-Reyes, Germán Buitrón, Iván Moreno-Andrade, Aida Cecilia Tapia-Rodríguez, Rodolfo Palomo-Briones, Elías Razo-Flores, Oscar Aguilar-Juárez, Jorge Arreola-Vargas, Nicolas Bernet, Adriana Ferreira Maluf Braga, Lucia Braga, Elena Castelló, Lucile Chatellard, Claudia Etchebehere, Laura Fuentes, Elizabeth León-Becerril, Hugo Oscar Méndez-Acosta, Gonzalo Ruiz-Filippi, Estela Tapia-Venegas, Eric Trably, Jorge Wenzel, Marcelo Zaiat, Standardized protocol for determination of biohydrogen potential, Methods X, Volume 7, 2020, 100754, https://doi.org/10.1016/j.mex.2019.11.027.
dc.identifier.urihttp://hdl.handle.net/11627/5592
dc.description.abstract"Biohydrogen production potential (BHP) depends on several factors like inoculum source, substrate, pH, among many others. Batch assays are the most common strategy to evaluate such parameters, where the comparison is a challenging task due to the different procedures used. The present method introduces the first internationally validated protocol, evaluated by 8 independent laboratories from 5 different countries, to assess the biohydrogen potential. As quality criteria, a coefficient of variation of the cumulative hydrogen production (Hmax) was defined to be <15 %. Two options to run BHP batch tests were proposed; a manual protocol with periodic measurements of biogas production, needing conventional laboratory materials and analytical equipment for biogas characterization; and an automatic protocol, which is run in a device developed for online measurements of low biogas production. The detailed procedures for both protocol options are presented, as well as data validating them. The validation showed acceptable repeatability and reproducibility, measured as intra- and inter-laboratory coefficient of variation, which can be reduced up to 9 %."
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBiohydrogen potential protocol
dc.subjectAutomatic protocol
dc.subjectDark fermentation
dc.subjectHeat-treated inoculum
dc.subjectManual protocol
dc.subject.classificationCIENCIAS TECNOLÓGICAS
dc.titleStandardized protocol for determination of biohydrogen potential
dc.typearticle
dc.identifier.doihttps://doi.org/10.1016/j.mex.2019.11.027
dc.rights.accessAcceso Abierto


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